重组人神经营养因子-4/5蛋白抗三氧化二砷神经毒作用  

Inhibitory Effects of Recombinant Human Neurotrophin-4/5 protein on Neurotoxicity Caused by Arsenic Trioxide

作　　者：楼屹[1] 王国荃[1] 黄燕[2] 殷明[2] 戴建凉 应康[2] 顾少华[2] 刘建平[2] 谢毅[2] 

机构地区：[1]新疆医学院预防医学系环境卫生教研室 [2]上海复旦大学遗传工程国家重点实验室

出　　处：《中华预防医学杂志》1999年第5期295-297,共3页Chinese Journal of Preventive Medicine

摘　　要：目的初步观察重组人神经营养因子-4／5（hNT－4／5）蛋白对三氧化二砷毒性的抑制作用。方法利用hNT－4／5蛋白具有抗神经毒性的特点，采用本实验室克隆表达及部分纯化的具有天然hNT－4／5蛋白生物学活性的重组hNT-4／5蛋白，以不同水平的重组hNT4／5蛋白（0－100μl）与不同浓度的As2O3（0－160μmol／L）同时加入各组鸡胚前脑神经细胞和PC12细胞培养液中共同孵育24－48小时，观察其对染毒鸡胚前脑神经细胞存活和PC12细胞突起生长的影响作用。结果在鸡胚前脑神经细胞和PC12细胞中与As2O3共同培养48小时后，对照组与实验组的细胞存活率差异有显著性，而且细胞存活率和突起数目随hNT－4／5浓度增高而提高和增加。结论初步观察到重组hNT4／5蛋白具有抑制As2O3的毒性作用，为从基因工程途径寻找抗环境毒物因子提供了依据。Objective To study preliminarily the inhibitory effects of recombinant human neurotrophin(hNT)-4/5 protein on neurotoxicity caused by arsenic trioxide (As2O3). Metheds Nerve cells of the chickenembryonic forebrain and PC12 cells were co-cultured with recombinant hNT-4/5 protein and As2O3(0-16 μmol/L)for 24 and 48 hours, respectively, to observe the survival of nerve cells and the outgrowth of PC12 cells, withclone expression and recombination techniques.Results There was significant difference in survival rate of nervecells betwwen the experimental and control groups(p<0.01)after co-culture for 48 hours, and survival rat andnumber of neuron outgrowth increased with the concentration of hNT-4/5 protein.Concusion Recombinant hNT4/5 protein could inhibit neurotoxicity caused by As2O3,which provided a basis to find an anti-toxic factor in theenvironment by genetic engiceering.

关 键 词：神经营养因子 hNT-4/5蛋白 三氧化二砷 神经毒 

分 类 号：R977[医药卫生—药品] R979.3[医药卫生—药学]