乙氧苯柳胺的高效液相电化学测定  

Determination of etofesalamide by high performance liquid chromatography with electrochemical detection

作　　者：周健[1] 李俊[2] 汤晓林[2] 金涌[2] 李常玉[2] 徐叔云[2] 

机构地区：[1]安徽医科大学口腔系,合肥230032 [2]安徽医科大学临床药理研究所,合肥230032

出　　处：《中国药理学通报》1999年第4期375-377,共3页Chinese Pharmacological Bulletin

摘　　要：目的　建立乙氧苯柳胺（ Ｅ Ｔ Ｏ Ｆ） 软膏皮肤用药后的血药浓度测定方法。方法　采用高效液相联用电化学检测器。结果　 Ｅ Ｔ Ｏ Ｆ 在６２５ ～１００ μｇ· Ｌ－ １ 呈线型关系，日间差５ ％～６ ％ ，日内差２１ ％ ～６１ ％ ，回收率８６ ％ ～９８ ％ ，最低检测限１２５ ｐｇ· Ｌ－ １ ． Ｅ Ｔ Ｏ Ｆ软膏（１ ｇ 含 Ｅ Ｔ Ｏ Ｆ１５０ ｍｇ） 分别涂在４ 名健康志愿者前臂屈侧４ ｃｍ ×４ ｃｍ 皮肤后，血液中 Ｅ Ｔ Ｏ Ｆ能很快被检出。结论　该方法灵敏度高，重现性好，误差小，能满足 Ｅ Ｔ ＯAIM To establish detecting method of etofesalamide(ETOF) in the healthy volunteers following skin application of ETOF ointment. METHODS High performance liquid chromatography with electrochemical detector. RESULTS The rank of ETOF from 6 25～100 μg·L -1 showed good correlation and the recovery rates extracted from plasma were in the range of 86%～98%. The variations of within day and day to day were 2 1%～6 0% and 5%～6 1% respectively. 125 pg·L -1 was minimal limit of detection. The plasma concentration was rapidly measured after ETOF ointment was spread on the skins of healthy volunteers. CONCLUSIONS The method is fit for pharmacokinetic study of ETOF in phase I clinical trial.

关 键 词：乙氧苯柳胺 高效液相色谱 电化学 药代动力学 

分 类 号：R986[医药卫生—药品] R969.1[医药卫生—药学]