猪FATP1基因5'调控区多态性及其与脂肪性状的相关分析  

Polymorphism in 5′regulatory region of porcine FATP1 gene and its association with fat traits

作　　者：华绪川[1,2] 张立凡[1] 蔡兆伟[1] 蒋晓玲[1] 徐宁迎[1] 张金枝[1] 

机构地区：[1]浙江大学动物科学学院,浙江杭州310029 [2]浙江省畜牧兽医局,浙江杭州310020

出　　处：《江苏农业学报》2011年第1期89-93,共5页Jiangsu Journal of Agricultural Sciences

基　　金：国家重点基础研究发展规划项目(2006CB102100);浙江省科技厅重大项目(2005C12005-2);浙江省博士后科研择优资助项目(2008X80801)

摘　　要：脂肪酸转运蛋白1(Fatty acid transport protein 1,FATP1)是脂肪酸转运蛋白家族(FATPs)的一员,具有促进脂肪细胞吸收利用长链和极长链脂肪酸的功能,在脂肪沉积过程中发挥着重要的作用。采用直接测序法寻找FATP1基因5'调控区+64～-723 bp片段的单核苷酸多态性,在-586 bp处发现了一个T→C突变SNP(单核苷酸多态性)座位,利用PCR-RFLP的方法检测它在3个试验猪群体中的基因型,发现该座位在皮特兰和金皮F2代猪群中存在多态性,而在金华猪中仅表现为FATP1g-586TFATP1g-586T基因型。建立混合模型方程组分析该座位对188头金皮F2代猪脂肪性状的效应,结果显示,猪FATP1基因第-586座位的不同基因型与肩背膘厚、6～7肋背膘厚、最后一根肋骨处背膘厚、平均背膘厚和板油重显著相关(P<0.05),表明FATP1基因5'调控区的第-586位上的T→C突变对猪脂肪沉积有一定的影响。Fatty acid transport protein 1（FATP1） is a member of FATPs,which plays an important biological role in cells by enhancing the uptake of long-chain and very long-chain fatty acids into cells for the utilization and storage of fat.In this study,partial 5′ flanking region（＋64—-723 bp） of the porcine FATP1 gene was sequenced and there was one single nucleotide polymorphism（SNP） at-586 bp.This mutation was T→C and it could be recognized by restriction endonuclease Hha I.The polymorphism of the FATP1 gene was detected using PCR-RFLP in pigs of Pietrain,Jinhua and Jinhua × Pietrain F2 resource family.The results showed the site polymorphisms（FATP1g-586CFATP1g-586T,FATP1g-586CFATP1g-586C and FATP1g-586TFATP1g-586T genotypes） in Pietrain and Jinhua × Pietrain F2 pigs,and only FATP1g-586TFATP1g-586T in Jinhua pigs.The effects of the SNP on fat traits in Jinhua × Pietrain F2 were estimated using a mixed model.Significant associations were found between different genotypes of g-586T→C with backfat thickness of shoulder,6-7 ribs and the last rib and their averages,as well as the leaf fat weight（P0.05）,which indicated that this mutation had certain effect on porcine fat storage.

关 键 词：猪 FATP1基因 脂肪性状 PCR-RFLP 

分 类 号：S828.2[农业科学—畜牧学]