从盾叶薯蓣组培苗中高压酸解制备薯蓣皂苷元  

Acidolysis Preparation of Diosgenin at High Pressure from the Cultured Plantlets of Dioscorea zingiberensis

作　　者：阴春晖[1] 李培琴[1] 赵江林[1] 单体江[1] 何桂玲[1] 周立刚[1] 

机构地区：[1]中国农业大学农学与生物技术学院,北京100193

出　　处：《天然产物研究与开发》2011年第1期114-117,共4页Natural Product Research and Development

基　　金：北京市自然科学基金(6092015);国家自然科学基金(30871662;31071710)

摘　　要：采用正交试验法对盾叶薯蓣(Dioscorea zingiberensis)组培苗中薯蓣皂苷元的高压酸解制备工艺进行了研究。以薯蓣皂苷元的含量作为评价指标,选用正交表L16(45),以样品用量、硫酸浓度、提取时间为因素,设计了3因素4水平的正交试验。结果表明:高压酸解提取薯蓣皂苷元的最佳工艺条件为:样品用量25 mg、硫酸浓度0.5 mol/L、提取时间2 h,在此条件下提取物中薯蓣皂苷元的平均含量为9.12 mg/g。Acidolysis preparation of diosgenin at high pressure from the cultured plantlets of Dioscorea zingiberensis was studied by L16 （4^5） orthogonal experiments. The effects of sample quantity,concentration of sulfuric acid, and extraction time were investigated. The results showed that diosgenin content of the plantlets reached 9.12 mg/g under the optimum extraction condition that was 25 mg of sample,0.5 mol/L of sulfuric acid, and 2 h of extraction time.

关 键 词：盾叶薯蓣 薯蓣皂苷元 高压酸解 正交试验 

分 类 号：R931.71[医药卫生—生药学] Q949.9[医药卫生—药学]