反相高效液相色谱法测定荧光增白剂CBS中N,N-二甲基甲酰胺的残留量  

Determination of residual N,N-dimethylformamide in fluorescent brightening agent CBS by reversed-phase high performance liquid chromatography

作　　者：甘宏宇 张天玉 

机构地区：[1]山西青山化工有限公司,山西运城044100

出　　处：《色谱》2011年第2期184-186,共3页Chinese Journal of Chromatography

摘　　要：建立了反相高效液相色谱外标法检测荧光增白剂CBS(即CF-351)中N,N-二甲基甲酰胺(DMF)残留量的方法。使用Accurasil C18(150 mm×4.6 mm,5μm)反相液相色谱柱,以甲醇-水为流动相梯度洗脱,流速为1.0mL/min,检测波长为205 nm,柱温为30℃,进样量为5μL。在上述条件下,在DMF质量浓度为0.19～141 mg/L范围内,其峰面积与质量浓度的线性关系良好。在加标水平(用质量分数表示)为0.234 4%和0.467 8%时,回收率为98.4%～107.3%。本方法最低检出限(LOD)为0.019%(质量分数),峰面积的相对标准偏差(RSD)为1.73%。此方法可快速、准确地测定出荧光增白剂CBS中DMF的残留量。A method for the determination of N,N-dimethylformamide （DMF） residue in fluorescent brightening agent CBS by reversed-phase high performance liquid chromatography （RP-HPLC） was established. An Ameritech Accurasil C18 column （150 mm×4.6 mm,5 μm） was used with methanol-water as the mobile phase with gradient elution. The flow rate of the mobile phase was 1. 0 mL/min and the detection wavelength was 205 nm. The column temperature was 30℃ and the injection volume was 5 μL. There was a good linear relationship between the peak area and the concentration of DMF in the range of 0. 19 - 141 mg/L. The recoveries of DMF were 98.4% - 107.3% at the spiked levels of 0. 234 4% and 0. 467 8%. The limit of detection was 0. 019%. The relative standard deviation was 1.73%. This method can be used for the determination of DMF in fluorescent brightening agent CBS quickly and accurately.

关 键 词：反相高效液相色谱 N N-二甲基甲酰胺 荧光增白剂CBS 残留量 

分 类 号：O658[理学—分析化学]