海参和海星脑苷脂对大鼠急性肝损伤影响的比较研究  

THE COMPARATIVE STUDY ON THE EFFECT OF CEREBROSIDE FROM SEA CUCUMBER AND STARFISH ON ACUTE LIVER INJURY IN RATS

作　　者：张蓓[1] 薛长湖[1] 周鑫[1] 杜磊[1] 胡晓倩[1] 王玉明[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,青岛266003

出　　处：《营养学报》2011年第1期19-23,共5页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30972285);国际科技合作项目(No.2010DFA31330)

摘　　要：目的比较海参和海星脑苷脂对四氯化碳致大鼠急性肝损伤的影响。方法 24只大鼠按体重随机分为4组,分正常组、模型组、海参脑苷脂组(SCC)和海星脑苷脂组(SF),分别腹腔注射相应受试物10天,并于实验进行第7天开始利用四氯化碳(CCl4)建立急性肝损伤模型。测定了血清肝损伤指示酶活性和肝脏氧化应激水平以及肝脂含量,并观察了肝脏形态学变化,进行了DNA电泳分析。结果与正常组比,模型组大鼠血清中肝损伤指示酶活性均显著升高,肝脏脂质大量蓄积,肝脏中丙二醛(MDA)和一氧化氮(NO)含量显著增加,谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性显著降低,肝脏呈现明显病变,肝细胞大量坏死。海参脑苷脂组血清乳酸脱氢酶(LDH)、丙氨酸转氨酶(或称谷丙转氨酶ALT or GPT)、碱性磷酸酶(AKP)和天冬氨酸转氨酶(或称谷草转氨酶,AST or GOT)活性和肝脏MDA、NO含量以及肝脂含量相比模型组显著降低,GSH-Px和CAT活性显著升高,肝组织病变和肝细胞坏死状况明显改善,而海星脑苷脂组血清酶活和肝脏氧化应激水平变化不显著,肝脏脂肪含量显著降低。肝组织病变和肝细胞坏死改善效果相比海参脑苷脂组略差。结论海参脑苷脂对四氯化碳引发大鼠急性肝损伤的机制可能与其能够清除自由基,降低过氧化物,调节脂质代谢等作用有关,而海星脑苷脂对大鼠肝脏脂质蓄积表现出良好的改善效果,但其对肝损伤的作用机制仍有待深入研究。Objective To compare the effect of cerebroside from sea cucuber and starfish on CCl4-induced acute liver injury in rats.Method Twenty four rats were randomly assigned into 4 groups： control group,model group,sea cucumber cerebroside group（SCC） and starfish cerebroside group（SF）.All rats were given intraperitoneal injection of corresponding samples for 10 d.At the 7 th day of the experiment,CCl4 was injected into the rats except the control group to establish the acute liver injury model.After 10 d feeding,the activities of serum alanine transaminase（ALT）,aspartate transaminase（AST）,alkaline phosphatase（AKP） and lactate dehydrogenase（LDH） indicating liver injury,the levels of hepatic oxidative stress and the hepatic lipid concentrations were detected,and the hepatic pathomorphologic changes were demonstrated by HE stain.The DNA electrophoresis was also analyzed.Results The serum enzyme activities indicating liver injury,the hepatic lipid,malondialdehyde（MDA） and nitric oxide（NO） contents in the model group were significantly increased as compared with the control,but significantly decreased the SCC group compared with the model.The activities of hepatic glutathione–peroxidase（GSH-Px） and catalase（CAT） were similarly strongly reduced in the model group as comparel to the control,but increased in the SCC group as compared with the model.Besides,the liver necrosis was observed extensively in the models,and improved in the SCC group.In the SF group,the activities of serum indicator enzymes and levels of hepatic oxidative stress were not obviously changed compared with the models,but the hepatic lipid contents were strongly reduced.The liver damage and necrosis were promoted not as well as the SCC group.Conclusion The mechanism of sea cucumber cereborside on the CCl4-induced liver injury might relate to its function of clearing free radicals,reducing oxide contents and regulating lipid metabolism,while the starfish cerebroside ameliorated the hepatic lipid accumulation,but it

关 键 词：肝损伤 脑苷脂 氧化应激 细胞坏死 

分 类 号：R285.5[医药卫生—中药学]