高粱DNA导入引起小麦HMW-GS的变异及其品质改良和变异机理分析  

Introducing Sorghum DNA into Spring Wheat:HMW-GS Variation,Quality Improvement and Its Mechanisms

作　　者：欧巧明[1] 倪建福[1] 崔文娟[1] 庞斌双[2] 

机构地区：[1]甘肃省农业科学院生物技术研究所,兰州730070 [2]北京市农林科学院杂交小麦工程技术研究中心,北京100089

出　　处：《中国粮油学报》2011年第1期15-19,共5页Journal of the Chinese Cereals and Oils Association

基　　金：甘肃省重大科技专项基金(0801NKDA015);甘肃省科技支撑计划[自然科学基金](0803RJZA041);甘肃省农科院农业科技创新专项基金(2009GAAS16)

摘　　要：采用花粉管通道法,将高粱(Sorghum bicolor L.)基因组DNA导入粉质籽粒的稳定品系89122中,后代经多代连续选择优良变异系,并进行了高分子质量麦谷蛋白亚基的SDS-PAGE电泳检测和品质化验分析。获得1个稳定遗传变异新品种,与导入受体89122相比,后代新品系2001502-23含有7+8、5+10优质亚基,HMW-GS发生明显变异,Gul D1位点基因发生等位变异,其表达产物由原来(89122)的2+12亚基为5+10亚基;其品质指标较受体得到改善,从而实现了受体小麦HMW-GS基因的遗传转化和品质改良。说明利用花粉管通道法完全可以实现小麦HMW-GS基因的转化和品质目标性状的遗传改良。而生物诱变是其可能的遗传转化和变异机理之一。The genome DNA of sorghum was introduced into floury grain spring wheat No.89122 via the pollen tube pathway.Excellent variant lines were chosen with multi-generation selecting.The detection of HMW-GS by SDS-PAGE electrophoresis and quality analysis were conducted.One stable variant was obtained.Compared with its recipient No.89122,the new variant 2001502-23 contained quality subunits 7＋8 and 5＋10,significant variation of HMW-GS occurred,gene alleles appeared in Gul D1 Locus,and HMW-GS 5＋10 were expressed in variants 2001502-23 while subunits 2＋12 were presented in its recipient No.89122.Its quality indexes were improved compared with the receptor.Thus the genetic transformation of HMW-GS gene and quality improvement for the receptor wheat was realized in this study.This result indicates that the genetic transformation of HMW-GS gene and aim character improvement of quality in wheat can be entirely achieved via the pollen tube pathway.In addition,the bio-inducted mutation is one of the mechanisms of its genetic transformation.

关 键 词：小麦 高粱DNA 花粉管通道法 高分子质量谷蛋白亚基(HMW-GS) 等位变异 

分 类 号：S512.332[农业科学—作物学]