米糠肽对D-半乳糖致衰小鼠线粒体损伤的影响  

Effect of Rice Bran Peptides on Mitochondria Injury in Aging Mice Induced by D-Galactose

作　　者：樊金娟[1] 付岩松[1] 宗立立[2] 张心昱[3] 罗霞[1] 

机构地区：[1]沈阳农业大学生物科学技术学院,沈阳110866 [2]吉林农业大学农学院,长春130118 [3]中国科学院地理科学与资源研究所,北京100101

出　　处：《中国粮油学报》2011年第1期30-34,共5页Journal of the Chinese Cereals and Oils Association

基　　金：辽宁省教育厅重点实验室项目(2008S207)

摘　　要：利用D-半乳糖(D-gal)致衰小鼠模型,探讨了米糠肽对小鼠心、脑线粒体锰超氧化物歧化酶(Mn-SOD)活性、丙二醛(MDA)含量以及总抗氧化能力(T-AOC)和Ca2+泵、Na+泵活性的影响,并以透射电镜观察了该肽对小鼠肝细胞及线粒体超微结构的影响。结果显示,与衰老组相比,高剂量米糠肽(500 mg/kg)可明显降低小鼠心、脑线粒体MDA含量(P<0.01),显著提高T-AOC(P<0.01)、Mn-SOD活性(P<0.01)以及Ca2+泵、Na+泵活性,优于中、低剂量(200、100 mg/kg);透射电镜下可见衰老组肝线粒体肿胀明显,膜模糊不清,有空泡形成,而米糠肽各组线粒体损伤程度有所减轻。说明米糠肽对D-gal致衰小鼠线粒体损伤起到良好的保护作用,可能具有延缓衰老的效果。The aging mice models induced by D-galactose（D-gal） were created to reveal the effect of rice bran peptides on the activity of manganese-superoxide dimutase（Mn-SOD）,content of malonadehide（MDA）,total antioxidation capacity（T-AOC）,and activities of Ca2＋-Mg2＋-ATPase and Na＋-K＋-ATPase in mice heart and brain mitochondria,as well as on the ultrastructure of liver cell and mitochondria under transmission electron microscopy（TEM）.Results：Compared with aging group,the rice bran peptides of 500 mg/kg dose greatly decrease the MDA content（P0.01）,significantly increase the T-AOC activity（P0.01）,and enhance the activities of Mn-SOD（P0.01）,Ca2＋-Mg2＋-ATPase and Na＋-K＋-ATPase in mice heart and brain mitochondria,and the effects in 500 mg/kg dose group is stronger than groups of 200 or 100 mg/kg dose.The liver mitochondria ultrastructure shows typical injury signs under TEM for aging group,whereas the mitochondria appearance of rice bran peptide groups shows much better.All these imply obvious protective and anti-aging effect of rice bran peptides on the aging mice induced by D-gal.

关 键 词：米糠肽 衰老模型 线粒体 超微结构 

分 类 号：TS2[轻工技术与工程—食品科学与工程]