野猪源与家猪源PRRSV陕西分离株全基因测序与遗传进化分析  

Genomic sequencing and phylogenetic analysis of PRRSV isolated from domestic pig and wild boar in Shaanxi Province

作　　者：张冲[1] 武小椿[1] 王波[1] 韩青松[1] 杨增岐[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2011年第2期9-16,22,共9页Journal of Northwest A&F University(Natural Science Edition)

基　　金：陕西省"13115"科技创新工程重大科技专项(2007ZDKG-13)

摘　　要：【目的】研究野猪源与家猪源猪繁殖与呼吸综合征病毒（Porcine reproductive and respiratory syn-drome virus,PRRSV）陕西分离株的遗传变异情况及分子生物学特征。【方法】根据VR-2332和HB-1（sh）/2002毒株序列设计引物,对2株PRRSV陕西分离毒株Shaanxi-1（家猪源）、Shaanxi-2（野猪源）全基因分段进行扩增并测序,将测序结果依次拼接得到病毒的全基因,然后进行序列的比对分析。【结果】PRRSV Shaanxi-1、Shaanxi-2毒株高度同源且均属于美洲型毒株,它们与2006年以前分离的毒株相比存在明显差异（与BJ-4株的核苷酸同源性仅为89%）,而与高致病性PRRSV（HP-PRRSV）保持较高的同源性（相似率为99%左右）。Shaanxi-1、Shaanxi-2分别呈现不同的变异特点,Shaanxi-1 ORF3、ORF5基因变异较大,ORF6、ORF7较为保守;Shaanxi-2 ORF7基因变异最大,ORF3~ORF6较为保守。分析发现,Shaanxi-1、Shaanxi-2具有HP-PRRSV的分子特征,但部分位点氨基酸的突变明显区别于HP-PRRSV。【结论】不同来源的Shaanxi-1、Shaanxi-2毒株均属于HP-PRRSV演化中的一个分支,但其发生了部分变异,使其具备了新的特点。【Objective】 The research was done to study genetic variation and molecular biological characteristics of PRRSV with different sources isolated from Shaanxi Province.【Method】 Primers were designed according to VR-2332 and HB-1（sh）/2002 strains,genome of two PRRSV strains Shaanxi-1（source of domestic pig）,Shaanxi-2（source of wild boar） isolated from Shaanxi Province was amplified and then sequenced,whole-genome sequence was obtained by splicing of correct sequencing and was aligned with other strains.【Result】 The results showed that Shaanxi-1 had a high homology with Shaanxi-2 and both of them belonged to American-type strain.Even though they had significant differences from the strains isolated before 2006（the homology only 89% compared with BJ-4）,they shared close to 99% homology with HP-PRRSV.However,Shaanxi-1,Shaanxi-2 had different variation characteristics,Shaanxi-1 ORF3,ORF5 varied greatly,ORF6,ORF7 revealed more conservative;Shaanxi-2 ORF7 got more variation relatively,ORF3~ORF6 seemed more conservative.Analysis showed that Shaanxi-1,Shaanxi-2 had common molecular characteristics with HP-PRRSV,but mutations of a few amino acids different from HP-PRRSV also existed.【Conclusion】 In summary,we could conclude that both Shaanxi-1,Shaanxi-2 from different sources are a branch of HP-PRRSV,however,part of the gene mutations enable them possess new features.

关 键 词：猪繁殖与呼吸综合征病毒 野猪与家猪 全基因测序 遗传进化分析 

分 类 号：S852.65[农业科学—基础兽医学]