扁吻鱼和塔里木裂腹鱼线粒体COI基因片段的比较研究  

Comparative Study on Partial Mitochondrial COI Gene of Aspiorhynchus laticeps and Schizothorax biddulphi

作　　者：杨天燕[1] 张人铭[1] 郭焱[1] 孟玮[1] 海萨[1] 马燕武[1] 

机构地区：[1]新疆维吾尔自治区水产科学研究所,新疆乌鲁木齐830000

出　　处：《水生态学杂志》2011年第1期45-50,共6页Journal of Hydroecology

基　　金：新疆维吾尔自治区自然科学基金项目(2009211A30);国家自然科学基金(31060356)

摘　　要：对扁吻鱼(Aspiorhynchus laticeps)和塔里木裂腹鱼(Schizothoraxbiddulphi)细胞色素c氧化酶亚基(COI)基因片段进行扩增。通过序列测定得到624bp的基因片段,无碱基的插入和缺失,A、T、G、C碱基中G含量最低,A+T含量高于G+C含量,与其它鱼类COI基因片段研究结果相一致。2个种间共检测到38处核苷酸替换,其中35处碱基转换,3处碱基颠换,蛋白质编码基因上的核苷酸替代主要是密码子第3位点上的同义替换。在编码的208个氨基酸中,仅发生2处氨基酸替代,替代率为0.96%。采用Kimura-2法计算种间平均遗传距离为0.06,以鲤作外群构建NJ系统树,扁吻鱼和塔里木裂腹鱼亲缘关系较近,而与新疆裸重唇鱼、斑重唇鱼亲缘关系较远。Mitochondrial cytochrome oxidase subunit I（COI）fragments of Aspiorhynchus laticeps and Schizothorax biddulphi were amplified.By sequencing,the sequence of 624 bp nucleotide was obtained,and no insertion/deletion was observed.Base G content was the lowest of all,and the content of A＋T was higher than that of G＋C,which was similar to the results in other fishes.38 nucleotide substitutions were checked between the two species which included 35 transitions and 3 transversions,and most of them were synonymous transitions at the third codon positions in the protein-coding genes.There were only two amino acid substitution in encoded 208 amino acids,and the substitution ratio was 0.96%.The average genetic distance by Kimura-2 method between A.laticeps and S.biddulphi was 0.06.Cyprinus cyprio was chosen as an out-group,and the NJ clustering tree indicated that A.laticeps and S.biddulphi had the closest relationship.

关 键 词：扁吻鱼 塔里木裂腹鱼 线粒体COI基因 

分 类 号：Q343.1[生物学—遗传学]