灵芝孢子油对MCF-7细胞凋亡及迁移的影响  

The Effects of Ganoderma Lucidum Spore Oil on MCF-7 Cell Apoptosis and Migration

作　　者：宋玉仙[1] 窦环[1] 刘贤钦[1] 公伟[1] 侯亚义[1] 

机构地区：[1]南京大学医学院免疫与生殖生物学实验室,江苏南京210093

出　　处：《中医药信息》2011年第2期91-94,共4页Information on Traditional Chinese Medicine

基　　金：南京大学研究生科研创新基金(2010CL04)

摘　　要：目的:观察灵芝孢子油对人乳腺癌MCF-7细胞增殖、迁移及凋亡的影响,并探讨其可能的作用机制。方法:CCK-8法检测灵芝孢子油对MCF-7增殖的影响;伤口愈合实验观察灵芝孢子油对MCF-7细胞迁移的作用;流式细胞术检测细胞凋亡率;RT-PCR法检测抗凋亡基因Bcl-2、促凋亡基因Bax及血管内皮生长因子(VEGF)mRNA表达水平。结果:不同浓度的灵芝孢子油对MCF-7的增殖和迁移均有一定程度的抑制作用,且呈时间、剂量依赖性;灵芝孢子油能显著诱导MCF-7细胞凋亡;RT-PCR结果显示,灵芝孢子油能上调Bax同时下调Bcl-2和VEGF的mRNA表达水平。结论:灵芝孢子油能抑制人乳腺癌MCF-7细胞增殖和迁移,并促进细胞凋亡,其作用机制可能与下调VEGF和Bcl-2/Bax的比值有关。Objective ：To observe the effects of Ganoderma lucidum spore oil on proliferation, apoptosis and migration of human breast cancer cell MCF - 7 in vitro, and explore its possible mechanisms. Methods ： The proliferation inhibition rate of MCF - 7 cells was determined by using the cell count kit 8 （ CCK - 8 ）. Wound healing assay was used to evaluate the cell migration. Cel] apoptosis was detected by flow cytometry. Furthermore, to analyze the expression level of Bcl - 2, Bax and VEGF mRNA, RT - PCR was performed. Results： Different concentrations of Ganoderma lucidum spore oil could inhibit MCF -7 cell proliferation as well as migration and took on dose and time dependently. It also induced cell apoptosis in a dose dependent way . The mRNA expressions of Bax related to apoptosis were increased, while Bcl -2 was decreased. On the other hand, the VEGF mRNA expression, which was important to tumor metastasis, was also declined. Conclusion ： Ganoderma lucidum spore oil can inhibit MCF -7 cell proliferation and migration, on the other hand, it induces cell apoptosis. The mechanisms may be partly due to the decreased Bcl -2/Bax rate and VEGF mRNA expression.

关 键 词：灵芝孢子油 MCF-7细胞 细胞增殖 细胞凋亡 细胞迁移 

分 类 号：R285.5[医药卫生—中药学]