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作 者:张传福[1] 闵小波[1] 柴立元[1] 钟海云[1]
出 处:《中南工业大学学报》1999年第5期489-492,共4页Journal of Central South University of Technology(Natural Science)
基 金:国家教育部博士点基金
摘 要:以溶液中Fe2+ 的氧化表征氧化亚铁硫杆菌的生长规律,研究了初始pH 值、温度、静置与振荡培养、接种量、接种状态以及雄黄、雌黄对细菌生长迟缓期长短的影响.研究结果表明:适宜的初始pH 值(2.0~2 .5) 、温度(30~35 ℃)、振荡培养、大的接种量以及处于对数生长期的菌种有相对较短的迟缓期;雄黄、雌黄的存在,延长了细菌生长的迟缓期,抑制了细菌的生长与繁殖.The influences of some factors, such as initial pH, temperature, steady state and shake culture, inoculation amount, inoculum state, and the existence of realgar and orpiment were examined on lag phase in growth of thiobacillus ferrooxidans, with the bacterial growth being expressed by the oxidation of ferrous iron in culture solution. It was indicated that lag phase is comparatively short under the condition of pH 2.0~2.5, temperature 30~35 ℃, shaking culture, great inoculation amount and inoculum in logarithmic phase. The existence of realgar and orpiment is harmful to the growth and prolongs the lag phase.
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