补肾健脾化瘀方药对老年小鼠免疫功能、自由基代谢及全血粘度的影响  

Study on the effects of kidney supplementing spleen invigorating stasis removing recipe on immune function, free radical metabolism and whole blood viscosity in old mice

作　　者：李志新[1] 王传社[1] 李顺成[1] 

机构地区：[1]北京医科大学中西医结合教研室,北京100083

出　　处：《北京医科大学学报》1999年第5期442-445,共4页Journal of Peking University(Health Sciences)

摘　　要：目的：为验证“正虚挟瘀”是中医衰老的重要原因／ 机制的可靠性及合理性，观察了补肾健脾化瘀天年安对老年小鼠免疫功能、自由基代谢及全血粘度的影响。方法：老年小鼠连续４ 周灌服天年安后，检测腹腔巨噬细胞产生的白细胞介素１（ ｉｎｔｅｒｌｅｕｋｉｎ１ ，ＩＬ１）、脾淋巴细胞产生的白细胞介素２（ｉｎｔｅｒｌｅｕｋｉｎ２，ＩＬ２） 的活性、全血粘度的变化、肝脑的脂质过氧化（ｌｉｐｉｄ ｐｅｒｏｘｉｄｅｓ，ＬＰＯ） 含量以及红细胞清除自由基酶的活性，如超氧化物歧化酶（ｓｕｐｅｒｏｘｉｄｅ ｄｉｓｍｕｔａｓｅ，ＳＯＤ） 、过氧化氢酶（ｃａｔａｌａｓｅ，ＣＡＴ） 、谷胱甘肽过氧化物酶（ｇｌｕｔａｔｈｉｏｎｅ ｐｅｒｏｘｉｄａｓｅ，ＧＳＨＰｘ）。结果：老年小鼠ＩＬ１、ＩＬ２ 的产生以及ＳＯＤ、ＣＡＴ、ＧＳＨＰｘ 均明显低于青年小鼠，而肝脑产生的ＬＰＯ 及全血粘度则明显增加。给药后，上述指标均可明显得到改善。结论：说明老年机体确“虚”和“瘀”并存，进一步为我们的观点提供了实验依据；同时也说明ＩＬ２ 的产生能力降低除与Ｔ 细胞本身有关外，还与巨噬细胞、自由基代谢等因素有关。Objective: In order to verify the reliability and reasonability of the theory that“deficiency of ZhengQi complicated with blood stasis” is the main cause/mechanism of aging. The effect of TianNianAn(TNA), a recipe of the kidney supplementing spleen invigorating blood stasis removing on immune function 、free radical metabolism and whole blood viscosity in old mice was studied. Methods: After old mice were medicated with TNA for 4 weeks, interleukin 2(IL 2) production of splenic lymphocytes, interleukin 1(IL 1) production of peritoneal macrophages, SOD, CAT and glutathione peroxidase(GSH Px) activities in erythrocytes, LPO contents in liver ( brain and the whole blood viscosity were detected. Results: The activities of IL 1, IL 2, SOD, CAT and GSH Px in old mice were significantly lower than those in the young mice, and LPO contents in liver and brain and the whole blood viscosity were higher. After medication of TNA, the above mentioned indices were obviously improved. Conclusion: The old organism really exists the constitution of “deficiency” and “stasis”, and further provides an experimental basis for our viewpoint. Meanwhile the decrease of IL 2 production was closely related with the function of macrophage and free radical metabolism, besides lower function of T lymphocyte.

关 键 词：补肾健脾化瘀药 免疫功能 自由基 血液粘度 衰老 

分 类 号：R285.5[医药卫生—中药学]