驯鹿的皮蝇蛆线粒体CO1基因序列分析  

Sequence analysis of mitochondrial cytochrome oxidase 1(CO1) gene of warble fly larvae from reindeer

作　　者：高慧[1] 杨晓野[1] 李云章[1] 莫内[1] 阿拉腾保力格 赵治国[1] 王瑞[1] 

机构地区：[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018 [2]内蒙古巴彦淖尔市乌拉特后旗潮格温都尔镇兽医站,内蒙古巴彦淖尔015500

出　　处：《畜牧与兽医》2011年第2期19-22,共4页Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(30760186)资助

摘　　要：利用分子生物学技术对内蒙古驯鹿未定种皮蝇蛆线粒体C01基因种属特异性序列进行了研究。DNA核苷酸测序结果证实：该种皮蝇线粒体C01种属特异性基因UEA7到UEA10特殊目标编码区域片段长度约为689bp；种系发生进化树和同源性分析显示其与鹿皮蝇法国株同源性非常接近，因此确定内蒙古地区感染驯鹿的皮蝇蛆为鹿皮蝇（Hypodermadiana）。不同蝇种间差异性分析显示：皮蝇科（Hypodermatidae）与狂蝇科（Oestridae）之间序列差异明显，差异性为19．3％～25．5％；寄生于鹿的不同蝇蛆种间差异性为1．9％～25．5％；皮蝇属（Hypoderrrta）不同种间差异性为6．7％～18．7％；鹿蝇属（Cephenemyla）不同种间差异性为1．9％～9．6％；而同种不同株的鹿皮蝇差异性为6．0％；说明生物线粒体CO1基因核苷酸序列在一定程度上可反映出种属及株间在进化上的差异性。The specificity of mitochondrial cytochrome oxidase 1 （ CO1 ） gene sequence of warble fly larvae of unknown species from reindeer in Inner Mongolia region of China were analyzed by molecular biological techniques. The results showed that the size of CO1 gene fragment from UEA7 to UEA10 was approximately 689 bp. The analysis of phylogenic tree showed that the unknown species from the reindeer in Inner Mongolia had high homology with Hypoderma diana France isolate. The differences analysis between the different fly species showed： the sequences between Hypodermatidae and Oestridae were obviously different, ranged from 19. 3% to 25.5%. The divergences of different fly larvae in deer ranged from 1.9% to 25.5%. The divergences of Hypoderma. ranged from 6. 7% to 18. 7%. The divergences of Cephenemyia. ranged from 1.9% to 9. 6% and the variation of the different Hypoderma diana strains was 6. 0%. It suggested that the nucleotide sequences of the mitochondrial CO1 genes could indicate the phylogenetic relationships among different genera, species and strains to some extent.

关 键 词：驯鹿 皮蝇 线粒体COI基因 进化树 序列比较 

分 类 号：S852.74[农业科学—基础兽医学]