纳米银抗菌活性和细胞毒性评价(英文)  

Evaluations of Antibacterial Activity and Cytotoxicity on Ag Nanoparticles

作　　者：李新平[1] 李胜利[2] 张淼涛[1] 张文龙[1] 李创宏[1] 

机构地区：[1]西北农林科技大学,陕西杨凌712100 [2]中国农业大学动物营养国家重点实验室,北京100083

出　　处：《稀有金属材料与工程》2011年第2期209-214,共6页Rare Metal Materials and Engineering

基　　金：National Natural Science Foundation of China (30471250)

摘　　要：以大肠杆菌和金黄色葡萄球菌做模型检测抗菌活性,选择L-929细胞通过MTT法评价毒性。结果证明纳米银具有显著的抗菌活性且呈剂量依赖性;不论在液体培养基还是固体培养基,30×10-6浓度产生的抗菌作用大于50%,但有潜在的细胞毒性;即使在3×10-6浓度下,细胞毒性达到2级,有26%～50%的细胞生长受到抑制。E.coli and S.aureus were used as model for testing antimicrobial activity,and L-929 cell line was selected for toxicity evaluation by cellular mitochondrial function（MTT assay）.The results show that silver nanoparticles（Ag NPs） can significantly in-crease antibacterial activity with the increasing of concentration of the silver nanoparticles.The lowest effective concentration of silver nanoparticles（30×10-6） which leads to more than 50% of inhibition in liquid medium and agar plate on bacterias is still satis-factory but will produce potential cytotoxicity.Even at 3×10-6 concentration of silver nanoparticles the cytotoxicity still exists with 2 grade which represents 26%-50% of cell growth inhibited,and 30×10-6 displays similar impact.

关 键 词：纳米银 大肠杆菌 金黄色葡萄球菌 抗菌活性 细胞毒性 

分 类 号：R114[医药卫生—卫生毒理学] TB383.1[医药卫生—公共卫生与预防医学]