HPLC法同时测定人参皂苷Rb_1、Rc、Rd、Rg_3、CK和Rh_2  

Qualitative and Quantitative Analysis of Ginsenoside Rb_1,Rc,Rd,Rg_3,CK and Rh_2 Simultaneously by HPLC

作　　者：李东霄[1] 常景玲[1] 梁刚[1] 李水水[1] 

机构地区：[1]河南科技学院生命科技学院,河南新乡453003

出　　处：《激光生物学报》2011年第1期87-93,107,共8页Acta Laser Biology Sinica

基　　金：河南省科技厅重点科技攻关项目(082102220001);河南省教育厅自然科学研究计划项目(2009B180006)

摘　　要：目的:建立高效液相色谱法同时测定人参皂苷Rb_1、Rc、Rd、Rg_3、CK和Rh_2的方法。方法:采用ODSC_(18)(4.6mm×150mm)色谱柱,流动相乙腈0.05%磷酸水,梯度洗脱,流速1mL/min,检测波长203nm,柱温35℃。结果:人参皂苷Rb_1、Rc、Rd、Rg_3、CK和Rh_2分离效果良好,线性关系良好,相对标准偏差和回收率符合2010年版中华人民共和国药典要求。结论:本方法简便、准确、稳定、重现性好,可用于上述人参皂苷的含量测定。Objective：To determine the content of ginsenoside Rbl, Rc, Rd, Rg3, CK and Rh2 simultaneously by HPLC. Methods：The samples were separated on an ODS C18 （4.6 mm × 150 mm） column using aeetonitrile-0.05 % phosphoric acid solution as the mobile phase, with the gradient elution at a flow of 1.0 mL/min and 35℃ column temperature. The wavelength of the detector was set at 203 nm. Results ： A good separation effect can be reached for ginscnoside Rb1 , Re, Rd, Rg3, CK and Rh2 which showed a good linear relationship between the peak area and the concentration of standard samples. The relative standard deviation （RSD） is good to meet the standard of pharmacopoeia of PRC. Conclusion：The method is simple, accurate, with a good reproducibility. It can be applied to qualitative and quantitative analysis of ginsenoside Rb1, Re, Rd, Rg3, CK and Rh2.

关 键 词：高效液相色谱法 人参皂苷RB1 人参皂苷Rc 人参皂苷RD 人参皂苷RG3 人参皂苷CK 人参皂苷RH2 

分 类 号：O657.32[理学—分析化学]