二阶导数光谱法测定溴吡斯的明聚乳酸微球的包封率  

Determination of entrapment efficiency of poly(DL-lactide) microspheres containing pyridostigmine bromide by second order derivative spectrophotometry

作　　者：徐美玲[1] 谭群友[2] 罗彦凤[3] 陶绍林[2] 张景勍[1] 

机构地区：[1]重庆医科大学高校药物工程研究中心和生物化学与分子药理重点实验室,重庆400016 [2]第三军医大学大坪医院野战外科研究所胸外科,重庆400042 [3]重庆大学生物工程学院生物材料与仿生工程中心,重庆400016

出　　处：《中国医院药学杂志》2011年第5期372-375,共4页Chinese Journal of Hospital Pharmacy

基　　金：教育部博士点基金资助项目(编号:20095503120008);重庆市教育委员会资助项目(首批高等学校优秀人才资助;编号:KJ090308)

摘　　要：目的：建立二阶导数光谱法测定溴吡斯的明聚乳酸微球的包封率。方法：通过离心法分离游离药物，采用二阶导数光谱法，于287nm波长处测定振幅D值，测定溴吡斯的明聚乳酸微球的包封率。结果：溴吡斯的明在16．68-38．92mg·L^-1范围呈良好线性关系；其回归方程为D=0．94×10^-2＋0．66×10^-2（r=（1．9998）；平均回收率100．30％、RSD为0．09％（n=3），24h内D值无明显变化，RSD为2．13％。结论：本方法操作简单、准确，可用于溴吡斯的明聚乳酸微球包封率的测定。OBJECTIVE To establish methods for the determination of the entrapment efficiency of polys（DL-lactide）microspheres containing pyridostigmine bromide. METHODS Centrifuging was applied to isolate free drug and second order derivative spectmphotometry was used. The peak amplitude was determined when the wavelength was at 287 nm. RESULTS The pyridostigmine bromide showed a good linear relationship within a range of 16. 68 - 38.92 mg· L^-1. The regression equation was D = 0. 94 × 10^-2 ＋ 0. 66 × 10^-2 （r = 0. 9998） ; the average recovery was 100. 30% and RSD = 0. 09% （n = 3）, numerical value D wasn＇t changed in the 24 h and RSD= 2.13%. CONCLUSION The method is simple, accurate and can be used for the determination of entrapment efficiency of poly（DL-lactide） mierospheres containing pyridostigmine bromide.

关 键 词：溴吡斯的明 微球 包封率 二阶导数光谱法 

分 类 号：R969.1[医药卫生—药理学]