好氧条件下复合生物膜-活性污泥反应器中的反硝化菌群结构  

Community structure of denitrifying bacteria in a hybrid AS-biofilm process under aerobic condition

作　　者：方芳[1] 王淑梅[1] 冯翠杰[1] 陈少华[1] 

机构地区：[1]中国科学院城市环境研究所,中国科学院城市环境与健康重点实验室,福建厦门361021

出　　处：《生态学杂志》2011年第3期430-437,共8页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(50778156);国家科技支撑计划项目(2009BAC57B02)资助

摘　　要：以nirK和nirS基因为标记,利用PCR-DGGE技术研究了好氧条件下复合生物膜-活性污泥反应器中的反硝化群落结构。结果表明,样品中大部分nirK-反硝化菌都属于α-变形菌纲中的根瘤菌目Rhizobiales,而nirS-反硝化菌则与β-变形菌纲(包括红环菌目Rhodo-cyclales和伯克氏菌目Burkholderiales)及γ-变形菌纲(假单胞菌目Pseudomonadales)细菌相似。在nirK-及nirS-群落中均发现了未与已知菌群聚类的特殊序列。细菌的生长方式(生物膜或活性污泥)对反硝化群落结构有显著影响。这些结果将为生物膜-活性污泥复合工艺中的反硝化过程提供基础数据。By using nirK and nirS as the markers,and with the methods of PCR and denaturing gradient gel electrophoresis（DGGE）,this paper studied the community structure of denitrifying bacteria in a hybrid AS-biofilm reactor under aerobic condition.In the samples collected,most of nirK-containing denitrifiers were belonged to the known Rhizobiales bacteria of α-proteobacteria,while the nirS-containing denitrifiers were similar to β-proteobacterium（Rhodocyclales and Burkholderiales）and γ-proteobacterium（Pseudomonadales）.Some specific sequences were found in nirS-and nirK-denitrifying communities.The data also demonstrated that the growth patterns of bacteria in biofilm or activated sludge could make a significant shift on the denitrifying community structure.These results would provide fundamental information for the understanding of the denitrificaiton in the hybrid AS-biofilm process under aerobic condition.

关 键 词：反硝化菌群落 功能基因 复合生物膜-活性污泥工艺 DGGE 

分 类 号：X703[环境科学与工程—环境工程]