双重保护培养SD大鼠骨髓来源树突状细胞  

Double protection for culturing SD rat bone marrow dendritic cells

作　　者：陶绍富[1] 李济宇[1] 

机构地区：[1]上海交通大学医学院附属新华医院普外科,200092

出　　处：《实用医学杂志》2011年第5期748-750,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:30600598);上海市科学技术委员会资助项目(编号:09411952500);上海市科学技术委员会资助项目(编号:10QH1401800);上海市教育委员会"曙光计划"资助项目(编号:10SG20)

摘　　要：目的:深入研究大鼠骨髓来源的树突状细胞的生物学功能,建立一种有效的细胞培养方法。方法:提取大鼠骨髓细胞,采用GM-CSF+IL-4进行体外诱导分化,对最终培养的细胞采用形态、表型、功能综合鉴定。结果:大鼠骨髓细胞培养12d,光镜下呈现典型的树突状细胞形态。细胞表面分子抗原随着培养时间的延长表达也增加。各时间点分子抗原表达差异有统计学意义(P<0.05)。其中第12天刺激T细胞增殖能力强。结论:该方法是一种经济、实用、有效的体外诱导扩增大鼠骨髓来源树突状细胞的培养方法。Objective To establish an effective cell culture method for further investigating the biological function of the rat bone marrow dendritic cells（BMDC）.Methods Rat BMDCs were induced by culture of rat bone marrow cells in the presence of both rrGM-CSF and rrIL-4 in vitro.The finally harvested cells were identified for morphology,phenotype and function.Results The final harvest cells displayed a typical dentritic cells morphology under the light microscope after 12 days of culture.The expression of the cell surface antigens increased in a time-dependent manner.Compared the molecular antigen expressions at different time,the difference was significantly（P 0.05）.The capacity of the cultured cells to stimulate reactive T cell proliferation reached peak on the twelfth day of the culture.Conclusion This culture method is economical,practical and effective for induction and expansion of rat BMDC in vitro.

关 键 词：树突细胞 大鼠 骨髓 

分 类 号：R392[医药卫生—免疫学]