雷公藤多苷对正常及佐剂关节炎大鼠睾丸组织形态结构、NOS表达影响的比较研究  

Comparative study on effect of poly glycosides of Tripterygium wilfordii Hook on morphology of testicles and expression of NOS in normal and adjuvant-induced arthritis rats

作　　者：李健[1,2] 谭勇[2] 吕永恒 何小鹃[2] 鞠大宏[4] 吕爱平[2] 

机构地区：[1]北京中医药大学基础医学院人体形态系,北京100029 [2]中国中医科学院中医临床基础医学研究所,北京100700 [3]天津市南开理疗医院,天津300100 [4]中国中医科学院中医基础理论研究所,北京100700

出　　处：《中国中医基础医学杂志》2011年第2期153-155,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金(30825047;30902000);"重大新药创制"科技重大专项(2009ZX09502-019)

摘　　要：目的:比较雷公藤多苷(GTW)对正常大鼠及佐剂关节炎雄性大鼠生殖毒性的差异。方法:雄性SD大鼠随机分为2组:正常组及弗氏完全佐剂诱导的关节炎组(每组60只)。各组大鼠同时以GTW灌胃,剂量分别为:0、7、70及105mg/kg/d,给药2周后取材,测量睾丸及附睾系数,检测睾丸组织中NOS含量及分布,观察睾丸组织病理变化。结果:105mg/kg GTW对正常及关节炎大鼠均能诱发生殖毒性;70mg/kg GTW可诱发正常大鼠生殖毒性,而该剂量GTW对关节炎大鼠没有明显的生殖毒性;7mg/kgGTW则对正常及关节炎大鼠均无生殖毒性。结论:GTW的睾丸毒性除与剂量相关外,与机体生理状态关联,相同剂量GTW在关节炎动物体内产生的睾丸毒性较正常动物弱。Objective：Comparative study on the differences of male reproductive toxicity disturbed by Tripterygium Wilfordii Multiglycoside（GTW） in normal rats and adjuvant induced arthritic rats（AA rat）.Methods：male SD rats were randomly divided into two groups,normal group and AA group.GTW 0,7,70 and 105 mg.kg-1 day-1 was given to rats intragastrically for two weeks.After that,all animals were sacrificed,the testicle and epididymal coefficient were calculated,histopathological changes of testis was observed by HE and NOS antibody immunohistochemical procedure,and the NOS level in testicle tissue was tested by chemical colorimetric method.Results：The changes were not detected both in normal group and AA group treated by 7mg.kg-1 GTW（clinical equal effective dosage）.70 mg.kg-1 GTW can induce testis toxicity in normal rats.However,none adverse impacts were observed in AA rats.105 mg.kg-1 GTW can arouse significant testis toxicity both in normal rats and AA rats.Conclusion：There is the dose-related feature on testis toxicity of GTW.On the other side,the testis toxicity is relative to physiological status.The same dosage GTW can rouse weak testis toxicity in AA rats than that in normal rats.

关 键 词：雷公藤多苷 SD大鼠 佐剂性关节炎 睾丸毒性 NOS 

分 类 号：R285.5[医药卫生—中药学]