Pb^(2+)对柄海鞘代谢的影响  

Effect of Pb^(2+) on Metabolism of the Ascidian Styela Clava

作　　者：姜爱莉[1] 于贞[1] 王长海[1] 

机构地区：[1]烟台大学海洋学院,山东烟台264005

出　　处：《北京理工大学学报》2011年第2期220-224,共5页Transactions of Beijing Institute of Technology

基　　金：国家自然科学基金资助项目(31070368);山东省优秀中青年科学家科研奖励基金计划项目(B52010HZ022)

摘　　要：应用半静态双箱式生物模型,考察Pb2+对柄海鞘代谢的影响.实验结果表明:0.01mg/L的Pb2+可促进柄海鞘的氨排泄,高浓度则使柄海鞘排氨率降低;0.01和0.05mg/L的Pb2+可以提高柄海鞘的清滤率,0.2mg/L则抑制其滤水作用;Pb2+对柄海鞘的氧氮比表现出与浓度相关的抑制作用.Pb2+影响柄海鞘几种代谢酶的活力,对碱性磷酸酶和Na+-K+-ATP酶活力均表现出与浓度相关的抑制作用;暴露于Pb2+溶液后,其SOD活力先降低后回升.以柄海鞘的代谢指标:氧氮比、碱性磷酸酶、Na+-K+-ATP酶和SOD活性作为海域污染的生物指标,具有一定的可行性.The effects of Pb2＋ on the metabolism of Styela clava were investigated using semi-static two compartment model.The results show that Pb2＋ could depress oxygen consumption rate of S.clava;0.01 mg/L Pb2＋ could improve its ammonia excretion rates,while the restrained effect occurrs when S.clava lies in higher concentration.Pb2＋ increases filtration rate of S.clava at the concentration of 0.01 and 0.05 mg/L,while it is controlled 0.2 mg/L.Restraining effect on O/N is correlative with Pb2＋ concentration.Pb2＋ could affect energy of metabolizing enzymes of S.clava.The depressing activities of alkaline phosphatase（AKP） as well as Na＋-K＋-ATPase are correlative with Pb2＋ concentration.When S.clava is exposed to Pb2＋,the activities of SOD descend immediately and then increase again.This study suggests that the metabolism parameters of S.clava（O/N,AKP,Na＋-K＋-ATPase and SOD） could be used as markers of heavy metal pollution in offing marine space.

关 键 词：柄海鞘 代谢 碱性磷酸酶 NA+-K+-ATP酶 SOD PB2+ 

分 类 号：Q178[生物学—水生生物学]