西黄丸及其拆方药味对斑马鱼胚胎血管生成的影响  

Effect of Xihuang Pills and its decomposed formula on zebrafish embryonic angiogenesis

作　　者：陈锡强[1,2] 侯海荣[1,2] 刘可春[1,2] 王思锋[1,2] 王希敏[1,2] 韩利文[1,2] 

机构地区：[1]山东省生物传感器重点实验室,山东济南250014 [2]山东省科学院生物研究所,山东济南250014

出　　处：《现代药物与临床》2011年第1期50-53,共4页Drugs & Clinic

基　　金：山东省科学院科技发展基金项目(SY2007BS10)

摘　　要：目的研究西黄丸中具有抑制血管生成活性的组方药味。方法采用发育时间为24h的血管荧光标记转基因斑马鱼胚胎作为模型,荧光显微镜下观察50μg/mL西黄丸甲醇提取物及其组方药味人工牛黄、人工麝香、乳香和没药甲醇提取物20μg/mL给药24h后对斑马鱼节间血管(IV)形成的影响。结果人工麝香、没药提取物对斑马鱼IV数量无影响,人工牛黄组IV数为22.67±2.60,抑制率为17.3%;乳香提取物组IV数分别为17.2±5.71,抑制率为37%;与对照组比较,人工牛黄、乳香提取物具有显著的抑制斑马鱼IV生成的作用(P<0.05或0.01)。结论乳香与人工牛黄是西黄丸中具有抑制血管生成作用的主要药味。Objective To study the component herbs for angiogenesis inhibition in Xihuang Pills. Methods Using 24 h vascular fluorescent transgenic zebrafish embryos as model, with fluorescent microscope the effects of 50 μg/mL Xihuang Pills＇ methanol extracts and four herbs methanol extracts which are artificial Bovis Calculus, artificial moschus, frankincense, and myrrh methanol （20μg/mL） on the zebrafish intersegmental vessels （IV） angiogenesis were observed in administration of 24 h.. Results Extract of artificial musk and myrrh had no effect on zebrafish IV angiogenesis, IV number in artificial Boris Calculus group was 22.67 a： 2.60, inhibition rates was 17.3%; IV number in frankincense group were 17.2 ± 5.71, inhibition rates was 37%. Artificial Bovis Calculus and frankincense could inhibit zebrafish IV angiogenesis significantly （P〈 0.05 or 0.01） compared with the non-treated group. Conclusion The frankincense and artificial Boris Calculus are main herbs in the Xihuang Pills that could inhibit angiogenesis.

关 键 词：西黄丸 拆方研究 斑马鱼 荧光标记 胚胎 血管生成 

分 类 号：R285.5[医药卫生—中药学] R293[医药卫生—中医学]