利用多重PCR探究男性尖锐湿疣与HPV感染之间的相关性  

The research on the relationship between condyloma and human papillomavirus with the multiplex PCR technology

作　　者：李付广[1] 丁显平[1] 朱一剑[1] 宋悦[1] 张敏 

机构地区：[1]四川大学生命科学学院遗传医学研究所,生物资源与生态环境教育部重点实验室,成都610064

出　　处：《四川大学学报（自然科学版）》2011年第1期225-230,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：重庆市医学科技重点项目(2009-2-084)

摘　　要：本文的主要目的是建立一种针对人乳头瘤病毒(Human Papillomavirus,HPV)检测的多重PCR方法,并利用这种方法探究男性尖锐湿疣与HPV感染之间的相关性.首先建立一种检测HPV的多重PCR方法,然后运用该方法对156例男性尖锐湿疣标本的HPV3种低危(HPV6,11,42)和6种高危(HPV16,18,33,52,56,58)亚型进行检测,并与反向膜杂交检测结果进行比较.结果156例标本中阳性标本数69例,占总标本数的44.23%,其中单一感染标本数48例,占69.57%,混合感染标本数21例,占30.43%.表明多重PCR技术在HPV检测方面具有较强的实用性;HPV6/11型感染与男性尖锐湿疣具有较高的相关性.To develop a multiplex PCR assay aiming at detecting human papillomavirus （HPV） infection, and to establish the prevalence of human papillomavirus HPV infection in men with condyloma. Methods： Of all, 156 male patients with condyloma were included in the present study. With the method of multiplex PCR, the samples were screened for HPV infection, including 3 kinds of low-risk （6, 11, 42） and six kinds of high-risk （16, 18, 33, 52, 56, 58） subtypes. And in the end, the results were further compared with those obtained from reverse cross-blot hybridization. In the 156 samples, 69 （44.23%） were detected positive for HPV infection, in which there were 69. 57%（48/69） single infection and 30. 43% （21/69） multiplex infection. Conclusion： Multiplex PCR technology in the HPV testing is strongly practical. There is a high prevalence of HPV6/11 subtype in men with eondyloma acuminata.

关 键 词：多重PCR 人乳头瘤状病毒 尖锐湿疣 

分 类 号：O781[理学—晶体学] R446[医药卫生—诊断学]