中度嗜盐菌H4的分离鉴定及硝酸盐还原能力分析  

Isolation and identification of mesohalophilic archaea H4 and analysis of the ability of nitrate reduction

作　　者：陈绍兴[1] 唐红斌[1] 杨权芬[1] 李沁[1] 陈娅[1] 苏嵘[1] 马自成[1] 谢志雄[2] 

机构地区：[1]红河学院生命科学与技术学院,蒙自661100 [2]武汉大学生命科学学院,武汉430072

出　　处：《四川大学学报（自然科学版）》2011年第1期231-236,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：红河学院生物化学与分子生物学重点学科建设项目(071010);红河学院校级课题项目(XJ1Y0802);云南省教育厅科学研究基金(09Y0413)

摘　　要：本研究采用嗜盐丰富培养基,通过富集培养的方法,从海南岛的东方盐场中分离到一株硝酸盐还原能力较强的嗜盐古菌菌株H4;形态观察结果显示,菌落呈橙红色、湿润、圆形;生理生化特征鉴定结果显示,其最适生长的盐浓度、pH值、温度分别为12%、6.5～7.5、38～40℃,并且生长需要Mg^(2+);能够厌氧生长;乳糖、半乳糖和核糖可以作为其生长的唯一碳源,脯氨酸和丙氨酸可以作为唯一氮源;采用MEGA4.0软件,并结合16S rDNA序列,进行系统发育树分析,确定H4菌株属于Haloferax属,并命名为Haloferax sp.H4.进一步对其硝酸盐还原能力进行定性和定量研究.A species of genus Haloferxa that grows under strict anaerobic conditions in the dark and requires Mg^2＋ , with strong nitrate reduction ability, was isolated from Eastern salt, one of the largest salt fields in Hainan Province, by the enrichment culture medium. Some morphological observations on this halobacteria showed that colonies were orange, moist and round; Physiological and biochemical charac- teristics suggested that the optimal temperature for grow was 38-40 ℃ and the optimal salinity at 12% and pH levels at 6.5-7.5. H4 could survive and grow under conditions of the lactose, galactose and ribose as a sole carbon source, and proline and alanine as a sole nitrogen source. A phylogenetic tree of halophilic archaea Halofera H4 was constructed by MEGA 4.0, based on the 16S rDNA sequence anal- ysis. Thus, we could identify it as genus of Haloferax and named Haloferax sp. H4. Furthermore, the qualitative and quantitative analysis of the reduction ability had been conducted.

关 键 词：中度嗜盐菌 HALOFERAX 硝酸盐还原 分离 鉴定 

分 类 号：Q93[生物学—微生物学]