不同产地野生与栽培地骨皮中香草酸含量的比较  

Comparison of the Contents of Vanillic Acid in Wild and Cultivated Cortex Lycii from Different Areas

作　　者：刘伟[1] 郭蕾[1] 崔永霞[1] 陈志红[1] 龚海燕[1] 

机构地区：[1]河南中医学院分析测试中心,郑州市450008

出　　处：《中国药房》2011年第11期1003-1004,共2页China Pharmacy

基　　金：河南省教育厅自然科学研究计划项目(2009A360010)

摘　　要：目的:比较不同产地栽培与野生地骨皮中香草酸的含量变化。方法:采用高效液相色谱法对野生与栽培地骨皮中香草酸的含量进行测定。色谱柱为Dikma Kromasil C18 100A(250mm×4.6mm,5μm),流动相为乙腈-1%冰醋酸水溶液(15∶85),检测波长为260nm。结果:香草酸的检测浓度在8.36～66.88μg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.9994);平均回收率为97.53%,RSD=1.51%(n=6)。栽培地骨皮中香草酸的含量整体高于野生地骨皮。结论:地骨皮的质量与其生长环境有密切关系。本试验结果可为地骨皮的开发利用提供科学依据。OBJECTIVE： To compare the change of the content of vanillic acid in wild and cultivated Cortex Lycii from different areas. METHODS： The content of vanillic acid in wild and cultivated Cortex Lycii was determined by HPLC. The determination was performed on Dikma Kromasil C18 100A （250 mm×4.6mm, 5 μm） column with mobile phase consisted of acetonitrile-1% glacial acetic acid solution （15：85） at detection wavelength of 260 nm. RESULTS： The linear range of vanillic was 8.36--66.88 μg.mL-1（r=0.999 4） with an average recovery of 97.53% （RSD=1.51%, n-6）. The content of vanillic acid in cultivated Cortex Lycii was higher than in wild Cortex Lyeii. CONCLUSION： The quality of Cortex Lycii is closely related to its ecological environ- ment. The study supplies scientific basis for the development and application of Cortex Lycii.

关 键 词：高效液相色谱法 地骨皮 香草酸 含量测定 野生 栽培 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]