参芦的质量标准研究  

Quality Standard of Ginseng Rhizome

作　　者：林世和[1] 易艳东[1] 余南才[1] 

机构地区：[1]武汉市第一医院中心实验室,武汉市430022

出　　处：《中国药房》2011年第11期1014-1016,共3页China Pharmacy

摘　　要：目的:建立参芦的质量标准。方法:根据2005年版《中国药典》方法,鉴别参芦的性状、显微特征,检查其水分、总灰分、酸不溶灰分;采用薄层色谱(TLC)法进行定性鉴别;采用高效液相色谱法测定人参皂苷Rg1、Re、Rb1的含量。结果:性状、显微特征明显;TLC斑点清晰、分离度好;人参皂苷Rg1、Re、Rb1的进样量分别在1.01～10.10(r=0.9997)、0.69～6.90(r=0.9994)、1.01～10.10(r=0.9998)μg范围内与各自峰面积积分值呈良好线性关系,平均回收率分别为99.91%、99.55%、99.65%,RSD分别为1.54%、1.67%、2.50%(n=9)。结论:所建标准可用于参芦饮片的质量控制。OBJECTIVE： To establish the quality standard for Ginseng Rhizome. METHODS： According to Chinese Pharmacopeia （2005 edition）, the property and microscopic characteristics of Ginseng Rhizome, moisture, total ashes content, acid-insolu- ble ashes content were identified by TLC. The contents of ginsenoside Rb~, Re and Rg~ in Ginseng Rhizome were determined by HPLC. RESULTS： The property and microscopic characteristics of Ginseng Rhizome were significant. The spot of TLC were clear and well-separated. The linear range of ginsenoside Rb~, Rg~ and Re were 1.01-10.10 （r=0.999 7） , 0.69-6.90 （r^-0.999 4） , 1.01-10.10（r=0.999 8）. The average recoveries were 99.91% ,99.55% ,99.65%, and RSD of them were 1.54%,1.67% ,2.50%（n=- 9）. CONCLUSION： The method is suitable for quality control of Ginseng Rhizome decoction pieces.

关 键 词：参芦 显微鉴别 薄层色谱法 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学] R927.1[医药卫生—中医学]