结核分枝杆菌抗原Rv1258c、Rv1410c及Rv3425的HLA限制性CTL表位预测  

Prediction of HLA restricted CTL epitopes in Mycobacterium tuberculosis antigens Rv1258c,Rv1410c and Rv3425

作　　者：朱宇皇[1] 高艳锋[1] 吕虹[1] 祁元明[1] 

机构地区：[1]郑州大学生物工程系,郑州450001

出　　处：《郑州大学学报（医学版）》2011年第1期13-15,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目30872381;河南省杰出人才基金资助项目084200510014

摘　　要：目的:预测结核分枝杆菌(MTB)相关抗原Rv1258c、Rv1410c及Rv3425的HLA限制性细胞毒性T细胞(CTL)表位。方法:通过NCBI数据库获取各蛋白的氨基酸序列,利用BLAST分析其与人类蛋白质的同源性,利用SYFPEITHI、BIMAS和NetCTL数据库预测分析MTB相关抗原的HLA-A2与HLA-A3限制性CTL表位。结果:初步筛选出3个候选抗原的HLA-A2和HLA-A3限制性CTL优势表位,分别为Rv1258c343～351、380～388、290～298、52～60;Rv1410c510～518、470～478、452～460、420～428;Rv3425118～126、43～51、29～37。结论:预测出MTB3个相关抗原的HLA-A2与HLA-A3限制性CTL表位,为抗结核多表位疫苗的研究奠定了基础。Aim：To predict the HLA restricted cytotoxic T lymphocyte（CTL）epitopes in Mycobacterium tuberculosis antigens Rv1258c,Rv1410c and Rv3425.Methods：The amino acid sequences of the antigens were obtained from NCBI database.Their homologies to human proteins were analyzed by BLAST.HLA-A2-and HLA-A3-restricted CTL epitopes were predicted by SYFPEITHI,BIMAS and NetCTL.Results：The predominant HLA-A2-and HLA-A3-restricted CTL epitopes predicted from the three candidate antigens were Rv1258c 343~351,380~388,290~298,52~60;Rv1410c 510~518,470~478,452~460,420~428;and Rv3425 118~126,43~51,29~37.Conclusion：Eleven HLA-A2-and HLA-A3-restricted CTL epitopes have been predicted from Mycobacterium tuberculosis antigens Rv1258c/Rv3425/Rv1410c,which could be used in research of antituberculosis multi-epitope vaccine.

关 键 词：结核分枝杆菌 Rv1258c Rv3425 Rv1410c 表位预测 

分 类 号：R52[医药卫生—内科学]