幽门螺杆菌尿素酶基因ureB植物双元表达载体的构建  

Construction of plant expression vector for ureB gene of Helicobacter pylori

作　　者：杨成运[1,2] 段广才[1,2] 范青堂[2] 

机构地区：[1]郑州大学公共卫生学院流行病学教研室,郑州450001 [2]河南省分子医学重点学科开放实验室,郑州450052

出　　处：《郑州大学学报（医学版）》2011年第1期57-59,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：中国博士后科学基金20070410252

摘　　要：目的:构建含有人幽门螺杆菌(H.pylori,Hp)ureB基因的植物表达载体。方法:采用高保真PCR技术从质粒pMED-ureB中扩增出ureB基因,构建质粒pUC18-ureB,再将pUC18-ureB酶切,将ureB基因与质粒pBI121连接。结果:构建的PBI121-ureB经PCR、酶切鉴定和测序分析证明,插入的基因片段为ureB基因,长度为1716bp,与GenBank报道的核苷酸序列同源性为99.76%,氨基酸序列同源性为100%。结论:成功构建了ureB基因的植物表达载体。Aim：To construct plant expression vector for Helicobacter pylori（HP）gene ureB.Methods：Gene ureB was amplified by high-fidelity PCR from plasmid pMED19-ureB and inserted into the corresponding endonuclease enzyme digested plasmid pUC18.The recombinant plasmid pUC18-ureB was digested and ureB gene was inserted into the plasmid pBI121.The recombinant vector pBI121-ureB was identified by PCR and restricted endonuclease enzyme.Results：Enzyme digestion analysis and sequencing results showed that the target gene was 1 716 bp and had been inserted into recombinant vector.Compared with gene reported by GenBank,the target gene had 99.76% homology in uncleotide acid sequence and 100% homology in amino acid sequenc.Conclusion：The plant expression vector of ureB has been constructed successfully.

关 键 词：幽门螺杆菌 UREB 植物表达载体 

分 类 号：R318[医药卫生—生物医学工程]