Immunosensor application based on Raman spectroscopy of porous silicon  

作　　者：江涛 莫家庆 吕小毅 晏鹏飞 贾振红 李江伟 张富春 

机构地区：[1]School of Physics, Xinjiang University [2]School of Electronic and Information Engineering, Xi'an Jiaotong University [3]Key Laboratory of Xinjiang Biological Resources and Gene Engineering, College of Life Sciences and Technology,Xinjiang University [4]College of Information Science and Engineering, Xinjiang University

出　　处：《Chinese Optics Letters》2011年第2期73-75,共3页中国光学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No. 60968002);the Program for New Century Excellent Talents in University of China (NCET-05-0897)

摘　　要：Porous silicon （PS） suitable for optical detection of immunoreaction is fabricated. The structure of immunosensor is prepared by the following steps： oxidization, silanization, glutaraldehyde cross-linker, and covalent binding of antibody. When antigen is added into the immunosensor, the Raman intensity is estimated to be linearly reduced according to the concentration of the surface protective antigen protein A （spaA） of below 4.0 μg ml-1. The ultimate detection limit is 1.412 × 102 pg ml-1. Controlled experiments are also presented with non-immune antigen of the spaA, and results show that the immunosensor has high specificity. Compared with the conventional enzyme-linked immuno sorbent assay （ELISA）, this method is quick, inexpensive, and label-free.Porous silicon （PS） suitable for optical detection of immunoreaction is fabricated. The structure of immunosensor is prepared by the following steps： oxidization, silanization, glutaraldehyde cross-linker, and covalent binding of antibody. When antigen is added into the immunosensor, the Raman intensity is estimated to be linearly reduced according to the concentration of the surface protective antigen protein A （spaA） of below 4.0 μg ml-1. The ultimate detection limit is 1.412 × 102 pg ml-1. Controlled experiments are also presented with non-immune antigen of the spaA, and results show that the immunosensor has high specificity. Compared with the conventional enzyme-linked immuno sorbent assay （ELISA）, this method is quick, inexpensive, and label-free.

关 键 词：ALDEHYDES ANTIGENS IMMUNOSENSORS Raman spectroscopy Sorption 

分 类 号：R392[医药卫生—免疫学]