Effects of Hydroxyapatite Nanoparticles on Apoptosis and Invasion of Human Renal Cell Carcinoma 786-0 Cells  

作　　者：WANG Zhi-xin HOU Yi HAN Wei WANG Kai-chen GUO Bao-feng LIU Ying CHANG Xi-hua WANG Wei-hua NA Wan-li KONG Xiang-bo ZHAO Xu ZHANG Ling 

机构地区：[1]Department of Urology, China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China [2]College of Chemistry [3]Department of Pathophysiology, Norman Bethune Medical School Jilin University, Changchun 130021, P. R. China

出　　处：《Chemical Research in Chinese Universities》2011年第1期94-98,共5页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China(Nos30801354 and 30970791);the Fundamental Research Funds for the Central Universities of China(No200812)

摘　　要：Renal cell carcinoma is the most common cancer of the kidney, and resistant to traditional therapies. The aim of this study is to investigate the effects of hydroxyapatite nanoparticles on human renal cell carcinoma 786-0 cells. Cell proliferation was assessed with an 3-（4,5-dimethylthiazol-2-yl） 2,5-diphenyltetrazolium bromide（MTT） staining kit. The apoptosis assay was assessed with an FITC Annexin V Apoptosis Detection Kit. Caspase-3 and caspase-12 were detected by immunocytochemical staining and semi-quantitative RT-PCR. Cell wound healing assay was used to ensure cell motility. Matrigel invasion assay was analysed via transwell chambers. Our results showed that hydroxyapatite nanoparticles significantly reduced cell proliferation, invasion and induced apoptosis of 786-0 cells. The inhibiting action may have relation with up-regulated caspase-12, leading the cells to apoptosis. This study suggests that hydroxyapatite nanoparticles may be an effective and delivery system for renal cell carcinoma therapy.Renal cell carcinoma is the most common cancer of the kidney, and resistant to traditional therapies. The aim of this study is to investigate the effects of hydroxyapatite nanoparticles on human renal cell carcinoma 786-0 cells. Cell proliferation was assessed with an 3-（4,5-dimethylthiazol-2-yl） 2,5-diphenyltetrazolium bromide（MTT） staining kit. The apoptosis assay was assessed with an FITC Annexin V Apoptosis Detection Kit. Caspase-3 and caspase-12 were detected by immunocytochemical staining and semi-quantitative RT-PCR. Cell wound healing assay was used to ensure cell motility. Matrigel invasion assay was analysed via transwell chambers. Our results showed that hydroxyapatite nanoparticles significantly reduced cell proliferation, invasion and induced apoptosis of 786-0 cells. The inhibiting action may have relation with up-regulated caspase-12, leading the cells to apoptosis. This study suggests that hydroxyapatite nanoparticles may be an effective and delivery system for renal cell carcinoma therapy.

关 键 词：Renal cell carcinoma Hydroxyapatite nanoparticle APOPTOSIS INVASION 

分 类 号：Q255[生物学—细胞生物学] TQ174.75[化学工程—陶瓷工业]