甲基苯丙胺中毒mG1uR5的表达(英文)  

Altered Expression of Metabolic Glutamate Receptor 5 inMethamphetamine Intoxication in the Rats

作　　者：张晶[1] 张浩[1,2] 曾晓锋[1] 王尚文[1] 赵永和[1] 李桢[1] 

机构地区：[1]昆明医学院法医学院,云南昆明650031 [2]中国云南省公安厅,云南昆明650228

出　　处：《现代生物医学进展》2011年第1期44-47,共4页Progress in Modern Biomedicine

基　　金：Natual Science of the country(30660202);Chun Hui item of ministry of education(Z2006-1-65003);Hubei province scientific deparment item(2006GH22)~~

摘　　要：目的:研究代谢性谷氨酸受体5(mG1uR5)在甲基苯丙胺中毒的损伤机制中的作用。方法:设立实验组,对照组。实验组分别给予20 mg/kg,10 mg/kg腹腔注射MA;对照组分别给予同剂量的生理盐水。末次注射后24 h内腹腔注射戊巴比妥钠麻醉大鼠后用4%多聚甲醛灌注、取脑后行代谢性谷氨酸受体5的免疫组织化学染色,观察并计数mG1uR5在不同脑区的表达。结果:实验组mG1uR5在大脑纹状体、海马的表达较对照组显著增强,差异有显著性(P<0.05),并呈剂量依赖性。结论:mG1uR5参与了甲基苯丙胺中毒的损伤机制。Objective： To investigate the effect of mG1uR5 receptors on methamphetamine hydrochloride in the rats.Methods Forty male Wistar rats were randomly divided into control group（n=20） and experimental group（n=20）.Rats in EG were peritoneal in-jection with methamphetamine hydrochloride（20 mg/kg-HDG,10 mg/kg-MDG,bid）.CG was administered with the same normal saline.Within 24 h of the last methamphetamine injection,rats were anesthetized by intraperitoneal injection of pentobarbital sodium and then were perfused by 4 % paraformaldehyde.After the removement of brain,mGluR5 by immunohistochemical staining were observed and counted in different brain areas.Results： The expression of mG1uR5 in the hippocampus and the striatum of the brain for experimental group was enhanced markedly compared with control group（P0.05） and it had dose dependent.Conclusion： MG1uR5 participated in the damage mechanism of methamphetamine poisoning.

关 键 词：甲基苯丙胺 中毒 mG1uR5 

分 类 号：Q95-3[生物学—动物学] R994.1[医药卫生—毒理学]