单细胞凝胶电泳分析地黄提取物对仓鼠肺成纤维细胞DNA的损伤作用  

Analysis on DNA damage of Chinese hamster lung fibroblas cell line induced by Rehmanniae Radix extract with single cell gel electrophoresis

作　　者：姜凌[1] 傅鹏[1] 张建军[1] 申秀萍[1] 

机构地区：[1]天津药物研究院新药评价中心,天津300193

出　　处：《药物评价研究》2011年第1期26-28,共3页Drug Evaluation Research

基　　金："十一五"国家科技支撑计划课题(2007BAI37B06)

摘　　要：目的采用单细胞凝胶电泳(彗星实验)技术研究地黄提取物致仓鼠肺成纤维细胞(CHL细胞)DNA的损伤作用,为该药物临床前安全性评价提供参考。方法采用0.2、1.0、5.0mg/mL地黄提取物,用双氧水作为阳性对照药,处理细胞,24h后收获细胞,进行彗星电泳实验。结果双氧水处理后造成CHL细胞产生明显的DNA损伤,呈现不同彗尾长度的彗星,与阴性对照组比较有显著差异。不同质量浓度的地黄提取物处理细胞后,彗星长、彗尾长度等各项指标与阴性对照组比较无显著差异。结论单细胞凝胶电泳检测DNA损伤的敏感度较高,不同质量浓度的地黄提取物对CHL细胞未产生DNA损伤。Objective To study the DNA damage of Chinese hamster lung（CHL） fibroblas cell line induced by Rehmanniae Radix extract using single cell gel electrophoresis（SCGE）（comet assay） so as to provide the reference for preclinical safety evaluation of the drug.Methods Taking H2O2 as positive drug,the cells were treated with Rehmanniae Radix extract at the concentrations of 0.2,1.0,and 5.0 mg/mL,respectively for 24 h,then the comet assays were performed.Results The obvious damage of CHL cells was induced by H2O2 and the significant difference was indicated by the the average tail length of comet in compared with the negative control group.There was no significant difference of every index,such as comet length and comet tail length at various concentrations of Rehmanniae Radix extract.Conclusion The comet assay shows considerable sensitivity to detect the lesion process.Different concentrations of Rehmanniae Radix extract could not induce DNA damage to CHL cells.

关 键 词：地黄 单细胞凝胶电泳 彗星实验 DNA损伤 肺成纤维细胞 双氧水 

分 类 号：R965.3[医药卫生—药理学]