RP-HPLC法同时测定加味黄龙颗粒中大黄酸、大黄素和大黄酚含量  

Simultaneous determination of Rhein、Emodin and Chrysophanol in the Huanglong particle by HPLC

作　　者：黄群[1] 林焕泽[1] 邱振文[2] 蓝忠[1] 李桃[1] 

机构地区：[1]茂名市人民医院,525000 [2]广州中医药大学第一附属医院

出　　处：《中国实用医药》2011年第5期7-9,共3页China Practical Medicine

摘　　要：目的建立高效液相色谱法同时测定黄龙颗粒中3种成分的含量。方法采用Hypersil ODS-C18柱（250 mm×4.6 mm,5μm）,甲醇-0.05%磷酸（85：15）为流动相,流速：0.8 ml/min,柱温：室温,检测波长：254 nm。结果大黄酸、大黄素和大黄酚在5.55-90.28μg/ml、5.24-87.54μg/ml和6.33-95.78μg/ml范围内呈良好的线性关系;相关系数分别0.9998、0.9999和0.9999;大黄酸、大黄素和大黄酚的平均加样回收率为98.17%（RSD=1.98%）、98.83%（RSD=2.05%）和96.81%（RSD=0.73%）。结论该方法简便、可靠、准确,可用于黄龙颗粒的质量控制。Objective To establish a HPLC method for the simultaneous determination of Rhein,Emodin and Chrysophanol in the Huanglong particle.Methods The determination was performed on Hypersil ODS-C18 column（250 mm×4.6 mm,5 μm）with mobile phase consisted of methanol-0.05%phosphoric acid（85：15）at a flow rate of 0.8 ml/min.The UV detection wavelength was set at 254 nm.Results A good linearity was found over the concentration range of 5.55~90.28 μg/ml（Rhein）、5.24~87.54 μg/ml（Emodin）and 6.33~95.78 μg/ml（Chrysophanol）.The correlation coeficient was 0.9998（Rhein）、0.9999（Emodin）and 0.9999（Chrysophanol）respectively.The average recovery of particle was 98.17%（RSD=1.98%）、98.83%（RSD=2.05%）and 96.81%（RSD=0.73%）.Conclusion The determination method is simple,reliable,accurate,and it can be used for quality control of Huanglong particle.

关 键 词：黄龙颗粒 质量控制 高效液相色谱法 

分 类 号：R286.0[医药卫生—中药学]