生远志及其总皂苷与蜜远志对胃肠Cajal间质细胞的影响  

Study of Cajal Interstitial Cells in Stomach and Small Intestine of Rats with Crude, Honey-stir-baked Radix Polygalae and its Saponins

作　　者：杨伟峰[1] 王建[2] 刘丽娜[2] 肖武[2] 

机构地区：[1]湖北中医药大学药学院,湖北武汉430065 [2]成都中医药大学药学院,四川成都610075

出　　处：《中药材》2011年第1期33-36,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(130371778)

摘　　要：目的:考察生远志及其总皂苷与蜜远志对大鼠胃肠Cajal间质细胞(ICC)的影响,探讨蜜炙降低生远志胃肠动力障碍的相关机制。方法:采用免疫组织化学染色法对比观察各组大鼠胃、小肠c-kit阳性ICC的变化。结果:生远志组和总皂苷组能显著降低胃、小肠肌间神经丛c-kit阳性ICC细胞的含量(P<0.01);而蜜远志组对胃、小肠肌间神经丛c-kit阳性ICC含量无显著影响(P>0.05)。结论:生远志与总皂苷导致胃肠动力障碍的机制,可能与减少胃肠ICC相关;远志总皂苷是引起胃动力异常的主要物质基础;远志经蜂蜜炮制后从某种程度上保护胃肠ICC。Objective ： To observe the changes of interstitial cells of Cajal （ICC） in stomach and small intestine of rats with honeystir-baked Radix Polygalae , crude Radix Polygalae and its saponins, so as to study mechanism of crude Radix Polygalae reducing the motility disorder in gastrointestinal tract. Methods ： Immunohistochemical staining was used to investigate the distribution of c-kit positive ICC in stomach and small intestine. Results ： Compared with control group, the c-kit positive ICC in stomach and small intestine of crude Radix Polygalae and its saponins groups were both markedly decreased in model group（both P 〈0. 01 ）,while honey-stir-baked Radix Polygalae group can not（ P 〉 0. 05 ）. Conclusion：The motility disorder in gastrointestinal tract caused by crude Radix Polygalae and its saponins may be associated with the changes of ICC number in stomach and small intestine, honey-stir-baked Radix Polygalae can protect ICC in some extent.

关 键 词：生远志及其皂苷 蜜远志 胃肠动力 CAJAL间质细胞 

分 类 号：R283[医药卫生—中药学]