MCP-1A-2518G基因多态性与2型糖尿病的相关研究  

Association between the A-2518G polymorphism in the monocyte chemoattractant protein-1 gene and type 2 diabetes mellitus

作　　者：于南南[1] 程晓亮[1] 李郑芳[1] 

机构地区：[1]昆明医学院第二附属医院内分泌科,云南昆明650101

出　　处：《云南医药》2011年第1期6-8,共3页Medicine and Pharmacy of Yunnan

摘　　要：目的探讨MCP-1A-2518G基因多态性在2型糖尿病的发生以及其血管并发症的发展中的作用。方法选择健康对照组55例,2型糖尿病患者135例,所有入选对象留取空腹静脉血,提取DNA,PCR扩增、酶切分析其基因型,应用病例-对照研究方法探讨MCP-1基因多态性与2型糖尿病及其并发症的关系。结果 (1)2型糖尿病患者MCP-1GG(-2518)基因型频率及G(-2518)等位基因频率显著高于正常对照组(P<0.01),差异有统计学意义。(2)2型糖尿病无血管并发症组与合并血管并发症组之间GG基因型及G等位基因频率差异无统计学意义(P>0.05)。结论在中国昆明汉族2型糖尿病人群中,MCP-1G(-2518)等位基因频率明显增高,MCP-1G(-2518)等位基因可能是2型糖尿病发生的危险因素,MCP-1A-2518-G基因多态性可能与2型糖尿病的发生有一定的相关性。Objective To approach the role of polymorphism of MCP-1 genes in the pathogenesis and development of type 2 diabetes mellitus（T2DM）and its macrovascular and microvascular complications. Methods We genotyped 55 normal subjects and 135 type 2 diabetes patients. All the subjects were drawed of fasting venous blood, then extracted genomic DNA.Genotypes of the MCP-1G（2518）A were performed by polymerase chain reaction followed by digestion with appropriate restriction endonucleases. The results of determination were analyzed statistically. Results （1） The frequencies of the GG（-2518） genotype and the frequencies of the MCP-1 G（-2518） allete were significantly higher in patients with T2DM compared with normal controls, and there was statistical significance（P〈0.01）. （2） There were no statistical significances for frequencies of the GG（-2518） genotype and the frequencies of the MCP-1 G （-2518） allete with diabetic complications compared to the uncomplicated（P〉0.05）. Conclusion The frequencies of MCP-l G （-2518） allele were significantly higher in the Han population of type 2 diabetes in Kunming, China. MCP-1 G（-2518） allete may be the risk factor of T2DM, and polymorphisms of MCP-1 genes may be associated with T2DM.

关 键 词：单核细胞趋化蛋白1 基因多态性 2型糖尿病 并发症 

分 类 号：R587.1[医药卫生—内分泌]