辐照处理对Bt水稻苗期生理生化特性的影响  

EFFECT OF IRRADIATION ON PHYSIOLOGICAL AND BIOCHEMICAL PROPERTIES OF BT RICE SEEDLINGS

作　　者：王忠华[1] 陈小坚[1] 包旭升[1] 陈玉玲[1] 顾勤琴[1] 

机构地区：[1]浙江万里学院生物技术研究所,浙江宁波315100

出　　处：《核农学报》2011年第1期174-178,共5页Journal of Nuclear Agricultural Sciences

基　　金：浙江省自然科学基金(Y306015)

摘　　要：分别以50、100、150、250和350Gy的60Coγ射线处理Bt水稻成熟干种子,将其培养成实生苗,进行苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)等的活性以及叶绿素含量、根系活力和种子萌发时淀粉酶活性的检测,并观察水稻苗期的生长状况,以研究不同辐射强度对Bt水稻苗期生长及生理生化特性的影响,确定Bt水稻诱变的适宜辐照剂量,为下一步开展Bt水稻诱变改良奠定基础。结果发现,在<250Gy剂量范围内,辐照处理对Bt水稻幼苗PAL、PPO、CAT、SOD等酶活性,叶绿素含量、根系活力以及种子萌发时淀粉酶活性的影响较小,与原亲本相似;从水稻苗期的生长状况来看,随着辐照剂量的增加(<250Gy),苗高有不断增高的趋势。综合辐照处理对Bt水稻苗期主要生理生化特性的影响,认为转Bt基因并不改变水稻的辐照敏感性。因此,可采用常规诱变处理方法进行Bt水稻的辐射育种研究。The seeds of two varieties of Bt rice were treated by 60↑Coγ-rays at the doses of 50, 100, 150, 250 and 350Gy, respectively, their original parent was used as control material. The seedlings cultured from above seeds were used to detect the root activity, seedling growth, chlorophyll content, activities of phenylalnine ammonialyase （PAL） , polyphenol oxidase （PPO）, catalase （CAT）, superoxide dismutase （SOD） and amylase to investigate the effect of irradiation treatment on the physiological and biochemical properties of Bt rice. The results showed that root activity, chlorophyll content, activities of PAL, PPO, CAT, SOD of Bt rice seedlings and amylase of germinating seeds were lower than those of the control group after irradiation treatment of 〈 250Gy, but the differences were not significant, which was similar to those of original parent. Meanwhile, it was found that with dose increasing, the seedling height was increased, suggesting that irradiation treatment could stimulate the seedling growth. Therefore, Bt transgene can not change the irradiation sensitivity of rice and the conventional method of rice can be used in Bt rice irradiation mutation breeding.

关 键 词：BT水稻 辐照处理 苗期 生理生化特性 

分 类 号：S511[农业科学—作物学]