Ⅲ型前列腺炎患者外周血中CD4＋CD25＋Treg及前列腺液中MCP-1的检测及意义  

Detection of peripheral blood CD4＋CD25＋requlatory T cells and the level of MCP-1 in EPS of patients with chronic non-bacterial prostatitis and its clinical significance

作　　者：李承勇[1] 兰晓煦[1] 陈明明[1] 武进峰[1] 李双平[1] 田智谋[1] 

机构地区：[1]山西医科大学第二附属医院泌尿外科,太原030001

出　　处：《中国男科学杂志》2011年第1期15-18,共4页Chinese Journal of Andrology

摘　　要：目的 探讨Ⅲ型前列腺炎/慢性骨盆底疼痛综合征（CAP/CPPS）患者外周血中CD4＋CD25＋调节性T细胞占CD4＋T细胞的比率以及检测其前列腺液（EPS）中单核细胞趋化蛋白-1（MCP-1）的水平,分析各检测指标与临床症状的相关性.方法 采用流式细胞仪检测48例CAP/CPPS患者和10例正常对照者外周血CD4＋CD25＋Treg占CD4＋T淋巴细胞的百分比;ELISA法检测两组受试者EPS中MOP-1水平.结果 CAP/CPPS组外周血中CD4＋T细胞及CD4＋CD25highTreg/CD4＋T细胞（28.12±4.32）%,（3.99±0.61）%与对照组（28.29±4.30）%（3.96±0.66）%相比;差异无统计学意义,P〉0.05.Ⅲ a组外周血中CD4＋T细胞及CD4＋CD25highTreg/CD4＋T细胞（28.33±4.35）%,（3.98±0.60）%与Ⅲb组（27.91±4.26）%（4.01±0.62）%相比;P〉0.05.Ⅲ型组CD4＋CD25＋Treg/CD4＋T细胞（6.48±1.34）%,低于对照组（14.66±2.16）%;P〈0.01.CAP/CPPS组外周血中CD4＋T细胞以及CD4＋CD25hignTreg/CD4＋T细胞与患者慢性前列腺炎症状指数评分（CPSI）均无相关性（P〉0.05）：CD4＋CD25＋Treg/CD4＋T细胞与患者疼痛评分呈负相关（r=-0.702,P〈0.05）.CAP/CPPS组外周血中CD4＋CD25＋Treg/CD4＋T细胞与EPS中MCP-1水平呈负相关（r=-0.682,p〉0.05）.CAP/CPPS患者前列腺液中MCP-1（0.45±0.09）ng/ml较对照组（0.18±0.02）ng/ml显著升高;P〈0.01.Ⅲ a组EPS中MCP-1水平（0.54±0.02）ng/ml较Ⅲb组（0.35±0.02）ng/ml显著升高;P〈0.01.CAP/CPPS组EPS中MCP-1水平与NIH-CPSI呈正相关（r=0.716,P〈0.01）,且与患者疼痛评分明显相关（r=0.875,P〈0.01）,CAP/CPPS患者前列腺液中MCP-1水平与EPS中白细胞数呈正相关（r=0.898,P〈0.01）.结论 Ⅲ型前列腺炎患者外周血中CD4＋CD25＋Treg数量表达下调,导致患者自身免疫反应增强,可能是CAP/CPPS的发病机制之一;MCP-1在CAP/CPPS的发病过程中起重要作用,并且与临床症状密切相关,MCP-1可能成为CAP/CPPS临床诊断的一个指标.Objective To investigate the significant of CD4＋CD25＋Treg in peripheral blood and MCP-1 in expressed prostatic secretion （EPS） in the pathogenesis of chronic non-bacterial prostatitis/chronic pelvic pain syn- drome （CAP/CPPS）. Methods The ratio of peripheral blood CD4＋C25＋ regulatory T cells was detected by flow cytometry from 48 CAP/CPPS patients and 10 normal controls. The level of MCP-1 in EPS was measured by ELISA. Results There was no significant difference between the（CAP/CPPS） patients and normal controls in the propotions of CD4＋T cells and CD4＋CD25highT regulatory cells in peripheral blood （P〉0.05）; The propotion of CD4＋CD25＋Tregulatory cells in peripheral blood were significantly lower than that of normal controls （/）〈0.05）; In addition, no significant correlation was found among the severity of symptoms （CPSI） and the propotions of CD4＋T cells and CD4＊CD25highT regulatory cells in peripheral blood; The levels of MCP-1 in EPS of patients with CAP/CPPS were markedly increased compared with that of controls （P〈0.05）. There was a positive correlation between the level of MCP-1 and NIH-CPSI. The negative correlations between the CD4＋CD25＋T regulatory cells and MCP-1 were found. There was also positive correlation between the MCP-1 and the pain index. In addition, The MCP-1 was positively correlated with WBC number in EPS. Conclusion The lower proportion of CD4＋CD25＋T regulatory cells in the patients with CAP/ CPPS which caused the increasing of autoimmunity, might be one of the mechanisms of CAP/CPPS. The MCP-1 might also be associated with the pathogenesis of CAP/CPPS.

关 键 词：前列腺炎 T淋巴细胞 调节性 趋化因子CCL2 

分 类 号：R697.33[医药卫生—泌尿科学]