黄芪对肺腺癌细胞生长抑制的实验研究  

作　　者：耿国军[1] 姜杰[1] 杜好信[1] 钱文轩[1] 张义[1] 杨丽华[2] 陈端扬[1] 陈隽鹏[1] 

机构地区：[1]福建省厦门市中医院,厦门361000 [2]厦门大学医学院,福建厦门361009

出　　处：《实用中西医结合临床》2011年第1期4-5,共2页Practical Clinical Journal of Integrated Traditional Chinese and Western Medicine

基　　金：福建省自然科学基金项目:闽科计[2008]59号文

摘　　要：目的:研究分化诱导剂黄芪(Astragalus)在体外对人肺腺癌SPC-A-1细胞生长抑制的影响。方法:用不同浓度的黄芪分别处理肺腺癌SPC-A-1细胞后,镜下观察癌细胞的生长情况;测定软琼脂克隆形成率;MTT(噻唑蓝)法测定生长抑制率。结果:不同浓度黄芪处理后细胞的生长明显受到抑制,细胞生长抑制率随黄芪的浓度增加而增加。结论:黄芪对肺腺癌SPC-A-1细胞的生长有明显的抑制作用。Objective：To study the activity of the growth suppression effects with Astragalus on SPC-A-1 human lung adenocarcinoma cel1.Methods：Different concentrations of Astragalus were used in the culture of SPC-A-1 cells.Determination of clone forming ability on soft agar and growth suppression effects with Astragalus were measured by a tetrazolium-based volorimetric assay（MTT assay）.Results：Proliferation of SPC-A-1 cells was inhibited effectively after being treated by different concentrations of Astragalus,the rate of growth suppression was increasing with increased concentration of Astragalus.Conclusion：Astragalus have the significant effect on inhibiting on SPC-A-1 human lung adenocarcinoma cel1.

关 键 词：黄芪 肺腺癌 SPC-A-1 MTT法 生长抑制 

分 类 号：R285.5[医药卫生—中药学]