脯氨酸与2,3-二氯-5,6-二氰-1,4-对苯醌荷移反应的研究  

Charge transfer reaction of proline with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone

作　　者：宋秀丽[1] 李省云[1] 白旭峰[1] 

机构地区：[1]太原师范学院化学系,太原030031

出　　处：《中国卫生检验杂志》2011年第2期300-301,共2页Chinese Journal of Health Laboratory Technology

基　　金：山西省高校科技开发项目(20080025)

摘　　要：目的：采用分光光度法研究了脯氨酸与2,3-二氯-5,6-二氰-1,4-对苯醌（DDQ）的荷移反应。方法：在常温下,脯氨酸与DDQ在十二烷基硫酸钠溶液及pH=10.0的三酸缓冲溶液中,反应10 min,可生成稳定的荷移络合物。结果：荷移络合物的组成比为1∶1,最大吸收波长λmax=523nm,表观摩尔吸光系数ε=1.60×103L.mol-1.cm-1,在10μg ml-1~70μg ml-1的范围内符合比尔定律,回收率在97.7%~100.5%的范围内,桑德尔灵敏度S=7.2×10-2μg cm-2,相对标准偏差不大于1.5%。结论：应用本方法测定脯氨酸样品的含量,结果与文献方法一致。Objective：A simple,quick and sensitive spectrophotometric method has been established for the determination of proline.Methods： It was based on a charge transfer reaction of studied drug with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone（DDQ） in Britton-Robinson（pH=10.0） buffer solution at room temperature for 10 min.Results： The composition of the complex of proline with DDQ was 1∶1.The apparent molar absorptivity of the complex was determined to be 1.6×103 L mol-1 cm-1 at 523 nm.The calibration curve was linear in the concentration range of 10 μg ml-1～70 μg ml-1 for proline.The relative standard deviation was less than 1.5% and the recovery was in the range of 97.7%~100.5%.Conclusion： This proposed method has been applied to the determination of proline in its pharmaceutical dosage forms with satisfactory results.

关 键 词：分光光度法 脯氨酸 2 3-二氯-5 6-二氰-1 4-对苯醌 荷移反应 

分 类 号：O657.32[理学—分析化学]