2006年-2009年广西水体及海水产品霍乱弧菌监测分析  

Surveillance on Vibrio Cholera isolated from water and aquatic products in Guangxi,2006-2009

作　　者：权怡[1] 方锦嵩[1] 周凌云[1] 林玫[1] 王鸣柳[1] 秦卫文[1] 

机构地区：[1]广西壮族自治区疾病预防控制中心,广西南宁530028

出　　处：《中国卫生检验杂志》2011年第2期464-465,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:通过水体及海水产品的霍乱弧菌监测,及时发现疫情传播来源,以有效控制霍乱疫情的发生。方法:采集各类海水产品分离培养霍乱弧菌,采用血清学、嗜菌体生物分型、药物敏感试验和分子生物学方法对不同来源的菌株进行鉴定。结果:2006年-2009年共分离出75株霍乱弧菌,其中小川型霍乱弧菌17株,稻叶型霍乱弧菌48株,O139群霍乱弧菌5株,首次分离出彦岛型霍乱弧菌5株。75株霍乱弧菌中仅3株O139群霍乱弧菌携带霍乱肠毒素(ctxA),其余菌株均为非流行株或非产毒株。药敏结果显示,除对氟哌酸、阿米卡星、环丙沙星、头孢他啶和头孢噻吩五种抗生素未产生耐药外,对庆大、复方新诺明、呋喃妥因、四环素、氨苄青霉素、强力霉素、氯霉素、链霉素和磺胺9种抗生素均产生不同程度的耐药。结论:广西外环境存在霍乱弧菌,但以非流行株或非产毒株为主,不同型别存在不同程度耐药性,未引起霍乱疫情的发生。Objective：To early detect the source and control the outbreaks via surveillance on Vibrio Cholera in aquatic products.Methods： Aquatic products were collected and cultured for Vibrio Cholera.The isolates from diverse sources were identified by serology,phage typing,antibiotic susceptibility tests and molecular biology.Results： A total of 75 strains of Vibrio Cholera were isolated between 2006 and 2009,of which,17 were identified Vibrio Cholera serotype Ogawa,48 Vibrio Cholera serotype Inaba and 5 Vibrio Cholera serogroup O139.Five strains of Vibrio Cholera serogtype Hikojima was detected for the first time.Cholera enterotoxin（ctxA）was detected in 3 strains of Vibrio Cholera O139 out of 75 strains isolated,while the remaining were non-toxigenic strains.The results of antimicrobial susceptibility tests showed that the strains were susceptible to norfloxacin,aminkacin,ciprofloxacin,ceftazidime and cefalothin,and were resistant to gentamycin,SMZ-Co,nitrofurantoin,tetracycline,ampicillin,doxycycline,chloramphenicol,streptomycin and sulfanilamide.Conclusion： Vibrio Cholera was detected in the environment in Guangxi.The vibrio isolated were mainly non-toxigenic strains.Resistance was found in different serotypes of Vibrio Cholera.No outbreak of cholera was observed in this area.

关 键 词：霍乱弧菌 药物敏感性 霍乱肠毒素 

分 类 号：R378.3[医药卫生—病原生物学]