卵形鲳鲹消化酶活性的研究Ⅳ 养殖水温和酶反应温度对幼鱼酶活性的影响  

On digestive enzyme activity in Trachinotus ovatusⅣ Effects of cultural and reactive temperatures on major enzyme activity in juveniles

作　　者：区又君[1] 罗奇[1,2] 李加儿[1] 蔡文超[1] 

机构地区：[1]中国水产科学研究院南海水产研究所,广州510300 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《海洋渔业》2011年第1期28-32,共5页Marine Fisheries

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(2008YD02)

摘　　要：比较研究了不同的养殖水温和酶反应温度对卵形鲳鲹幼鱼（体重为0.82±0.15 g）消化酶活性的影响。在第一组试验,将卵形鲳鲹幼鱼分别在19℃、22℃、25℃、28℃和31℃各温度下养殖7 d,然后在相同的酶反应温度下测定其消化酶活性;第二组试验,将卵形鲳鲹幼鱼在25~26℃驯养7 d,然后分别在15℃、20℃、25℃、30℃、35℃、40℃、45℃和50℃的酶反应温度下测定其消化酶活性。结果表明,在19~31℃养殖水温下,卵形鲳鲹幼鱼蛋白酶、淀粉酶、脂肪酶活性随水温上升均呈先上升后下降的趋势;蛋白酶活性在28℃时最高,淀粉酶活性在25℃时最高,脂肪酶活性在22℃时最高。在15~50℃的酶反应温度范围内,卵形鲳鲹幼鱼蛋白酶、淀粉酶、脂肪酶活性随温度上升均呈先上升后下降的趋势;蛋白酶活性在40℃时最高,淀粉酶活性在35℃时最高,脂肪酶活性在40℃时最高;与养殖水温相比,其最适反应温度明显偏高。The variation of activity of three digestive enzymes in juvenile Trachinotus ovatus with body weight of 0.82±0.15 g was investigated under different cultural and reactive temperatures.In the first group,juveniles were acclimated at five different water temperatures（19 ℃,22 ℃,25 ℃,28 ℃ and 31 ℃） for 7 days,respectively.After starvation for 24 hours,fish samples were seleted randomly and enzyme activity were dissected at the same reactive temperature.In the second group,juveniles were acclimated at the natural temperatures of 25-26℃ for 7 days.Enzyme activity were measured at eight different reactive temperatures（15 ℃,20 ℃,25 ℃,30 ℃,35 ℃,40 ℃,45 ℃ and 50 ℃）.Results showed that the enzyme activity increased with the cultural temperatures increasing and then decreased in the range from 19 to 31 ℃.The optimum cultural temperatures for protease,amylase and lipase were 28℃,25℃ and 22℃,respectively.The activity of three digestive enzymes increased with the reactive temperatures increasing and then decreased in the range from 15 to 50 ℃.The optimum reactive temperatures for protease,amylase and lipase were 40 ℃,35 ℃and 40 ℃,respectively.The optimum reactive temperatures were obviously higher than the cultural temperatures.

关 键 词：卵形鲳鲹 幼鱼 消化酶 养殖水温 酶反应温度 

分 类 号：Q55[生物学—生物化学]