转反义PLDγ基因小麦的耐盐性鉴定  

Salt Resistance of Transgenic Wheat with Anti-PLDγ Gene

作　　者：王瑞霞[1] 高庆荣[2] 钱兆国[1] 丛新军 米勇[1] 牟秋焕[1] 孙宪印[1] 王超[1] 

机构地区：[1]泰安市农业科学研究院,山东泰安271000 [2]山东农业大学农学院

出　　处：《安徽农学通报》2011年第4期32-33,116,共3页Anhui Agricultural Science Bulletin

基　　金：现代农业产业技术体系(nycytx-03);公益性行业(农业)科研专项经费项目(201003002-08)

摘　　要：利用穗茎注射法将反义PLDγ基因导入普通小麦兰考906,对经特异PCR扩增和PCR-Southern杂交技术筛选出的转基因植株及其受体进行了耐盐性鉴定。耐盐水培鉴定和0.5%的混合盐土培鉴定结果及差异性检验表明,转基因株系的耐盐性均达到1级或2级水平,明显的优于受体。Antisense phospholipase Dγ（PLDγ） gene was introduced into LK906 wheat mediated by earstem injecting of Agrobacterium.The salt resistance variation were analyzed for transgenic wheat detected by PCR and PCR-Southern technique.There was distinct difference between transgenic lines and receptor in salt resistance.Through the identification of salt resistance in water solution and 0.5% mixed salt resistance in soil and the test of variance,the result showed that transgenic lines＇ degree of salt resistance were 1 or 2,which was better than receptor significantly.

关 键 词：穗茎注射法 转基因小麦 耐盐 

分 类 号：Q964.5[生物学—昆虫学] S512[农业科学—作物学]