蛋白酶体抑制剂对人晶状体上皮细胞的增殖抑制作用  

Inhibitory effect of proteasome inhibitor on the proliferation of human lens epithelial cells

作　　者：赵玉新[1] 王昭霞[1] 王学红[1] 田霞[1] 宋钰[1] 葛建杰[1] 吴明星[2] 

机构地区：[1]威海文登中心医院眼科,山东省威海市264400 [2]广东省眼科学国家重点实验室中山大学中山眼科中心

出　　处：《中国医师杂志》2010年第12期1585-1589,共5页Journal of Chinese Physician

基　　金：国家自然科学基金资助（编号：30572003）

摘　　要：目的 探讨蛋白酶体抑制剂MG132对SRA01/04细胞增殖的影响.方法 以不同浓度MG132处理SRA01/04细胞36 h,通过MTT微量比色法检测MG132对SRA01/04细胞活力的影响,通过流式细胞术（FCM）检测MG132对SRA01/04细胞凋亡及细胞周期的影响,通过AnnexinV/FITC-PI双染法观察MG132对SRA01/04细胞的影响.结果 随着浓度的提高（0、0.1、0.5、1.0、2.5、5.0、10.0 μmol/L）,MG132对SRA01/04细胞增殖的抑制作用逐渐增强.36 h时,半数有效抑制浓度IC50为2.50μmol/L.FCM检测细胞凋亡结果：2.5、5.0 μmol/L的MG132作用于SRA01/04细胞36 h,细胞早期凋亡率分别为（6.55±0.35）%和（13.75±3.18）%,而对照组为（0.75±0.21）%,差异有统计学意义（P＜0.01）.2.5、5.0 μmol/L MG132处理SAR01/04细胞48 h,G0/G1期细胞比例分别为（73.42±3.10）%,（80.95%±3.83）%,而对照组为（42.57±0.64）%,差异有统计学意义（P＜0.01）;S期细胞比例分别为（17.40±1.50）%,（19.57±1.29）%,而对照组为（49.44±1.36）%,差异有统计学意义（P＜0.01）.免疫荧光镜下,MG132诱导SRA01/04细胞的早期凋亡.结论 蛋白酶体抑制剂MG132诱导细胞凋亡、影响细胞周期来抑制SRA01/04细胞的增殖.蛋白酶体抑制剂可起到防治后发性白内障的作用.Objective To investigate the effect of proteasome inhibitor MG132 on the proliferation of human lens epithelial cells SRA01/04. Methods The SRA01/04 cells were treated with MG132 by different concentrations （0, 0. 1, 0. 5, 1. 0, 2. 5, 5.0, 10. 0μmol/L） for 36 hours. The cell viability in all groups was determined using methylthiazoltetrazolium （MTT） test. The effect of MG132 on the apoptosis and regulation of cell cycle about SRA01/04 cells were detected by flow cytometry （FCM）. The SRA01/04 cells treated with MG132 were observed after Annexin V/FITC-PI staining by fluorescence microscope. Results The inhibitory effect of MG132 on SRA01/04 cells proliferation was enhanced with the increase of MG132 concentration. The 50% inhibiting concentration （ IC50 ） of MG132 was 2. 50μmol/L after SRA01/04 cells were treated with MG132 for 36 hours. The apoptosis index of the cells treated by MG132 at 2. 5μmol/L and 5 μmol/L for 36 hours was 6. 55 ± 0. 35% and 13.75 ± 3.18%, and 0. 75 ± 0. 21% for 5.0μmol/L for 36 hours in control group. After cells were treated with MG132 for 48h, the percentages of cells at G0/G1 phase were （42. 57 ± 0. 64） %, （73.42 ± 3.10） %, （ 80. 95 ± 3.83 ） % 0, 2. 5,5.0 μmol/Lgroups respectively, and those at S phase were （49. 44±1.36）%, （ 17. 40 ± 1.50）%, （ 19. 57 ± 1.29）%.Annexin V/FITC-PI staining was used, and MG132 was found to result to apoptosis. Conclusions MG132 could inhibit the proliferation of SRA01/04 cells by the effect of inducing apoptosis and regulation of cell cycle. The proteasome inhibitor-might play a key role in the prevention of posterior capsular opacification.

关 键 词：蛋白酶体内肽酶复合物/拮抗剂和抑制剂 晶体/细胞学/病理学 上皮细胞/药物 作用/病理学 细胞增殖/药物作用 

分 类 号：R7[医药卫生—临床医学]