杜氏利什曼原虫中国山丘疫区分离株磷脂多糖1(LPG1)基因及两侧非编码区序列的获取及分析  

Acquisition and Analysis of Lipophosphoglycan 1 Gene and the Noncoding Region of Leishmania donovani Isolates from Hilly Foci of China

作　　者：陆小军[1] 严可宁[1] 简君[1] 杜晓青[1] 马莹[1] 

机构地区：[1]四川大学华西医院实验医学科,成都610041

出　　处：《中国寄生虫学与寄生虫病杂志》2010年第6期465-467,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学基金(No.30500422)~~

摘　　要：通过PCR技术获得杜氏利什曼原虫中国山丘疫区分离株磷脂多糖1(LPG1)基因上游序列约2.2 kb,对该序列测序,根据测得序列设计引物,以染色体步移技术获得该序列下游约2 kb的核苷酸序列。将上述所获的2个序列拼接获得杜氏利什曼原虫中国山丘疫区分离株LPG1基因及其两侧非编码区核苷酸序列4 121 bp(GenBank登录号为HMO27899)。该序列与GenBank中相关序列进行序列一致性分析,结果显示,LPG1基因与多种利什曼原虫LPG1基因的核苷酸序列一致性为72%～78%。A fragment about 2.2 kb located at upstream of lipophosphoglycan 1 （LPG1）gene of Leishmania donovani isolate from hilly foci of China was obtained by PCR.The nucleotide sequence of the fragment was determined by sequencing.The sequence of the LPG1 gene and its downstream fragment （about 2 kb）was determined by using genome walking method.The above two fragment splicing result showed that the nucleotide sequence of the LPG1 gene with the noncoding region was 4 121 bp （GenBank accession number：HMO27899）.The BLAST results showed that the LPG1 geue of L.donovani isolate from hilly foci of China had 72%-78% nucleotide identity compared to that of other Leishmania spp. in GenBank.

关 键 词：杜氏利什曼原虫 磷脂多糖 染色体步移技术 

分 类 号：R382.22[医药卫生—医学寄生虫学]