糖尿病大鼠肾内小动脉早期动脉粥样硬化的实验研究  

Experimental Study of Early Atherosclerosis in Renal Arteriole of Diabetes Mellitus Rats.

作　　者：欧三桃[1] 刘琦[1] 张帆[1] 李莹[1] 

机构地区：[1]泸州医学院附属医院肾病内科,四川省泸州市646000

出　　处：《医学理论与实践》2011年第4期373-374,377,共3页The Journal of Medical Theory and Practice

基　　金：四川省卫生厅基金(川卫办发[2008]527号)

摘　　要：目的:探讨糖尿病大鼠大血管发生动脉粥样硬化(AS)时,肾内小动脉上有无MCP-1、ICAM-1等早期促AS形成的细胞因子的表达。方法:建立链脲佐菌素(STZ)诱导的糖尿病大鼠模型加高脂、高糖饲养,12周时处死大鼠,HE染色行大鼠主动脉、肾组织病理组织形态检查,免疫组化方法检测MCP-1、ICAM-1表达。结果:DM组血脂、血肌酐及尿蛋白较对照组明显增高。DM大鼠主动脉出现明显动脉粥样硬化改变,同时肾内小动脉上出现MCP-1、ICAM-1的表达,且与血肌酐、尿蛋白水平呈正相关。结论:糖尿病大鼠主动脉发生AS时,肾内小动脉上已有MCP-1、ICAM-1等早期促AS形成的细胞因子的表达,可能参与了糖尿病肾病的发生、发展。Objective：To observe the expression of monocyte chemotactic protein-1（MCP-1）and intercellular cell adhesion molecule-1（ICAM-1） in renal arteriole.Methods：Forty adult Sprague-Dawley male rats were randomly devided to two group：control group and DM group.High fat and high glucose diet was used to establish a DM rat mode1.Paraffin sections of renal tissue of rats were stained by routine methods and immunohistochemistry.Results：The levels of serum lipid,creatine and urine protein were markedly higher than that of control group.MCP-1 and ICAM-1 have expressed in renal arteriole when the formation of atherosclerosis in aorta.The expression of MCP-1and ICAM-1 related to serum creatine,urine protein positively.Conclusion：MCP-1 and ICAM-1 have expressed in renal arteriole when the formation of atherosclerosis in aorta of diabetes mellitus rats.It may indicate that they participate in the development of diabetic nephropathy.

关 键 词：糖尿病 动脉粥样硬化 单核细胞趋化蛋白-1 细胞间黏附分子-1 

分 类 号：R587.2[医药卫生—内分泌]