甲型流感病毒感染致人血管内皮细胞凋亡的初步研究  

Preliminary Study of H1N1 Influenza Virus Infection on the Apoptosis in Human Endothelial Cells

作　　者：倪念亲 石文松[2] 陈进 王娟娟 

机构地区：[1]湖南省株洲市石峰区疾病预防控制中心,湖南株洲412005 [2]株洲市疾病预防控制中心

出　　处：《实用预防医学》2011年第2期221-224,共4页Practical Preventive Medicine

摘　　要：目的探讨甲型流感病毒感染对血管内皮细胞增殖与凋亡的影响。方法 用纯化的甲型流感病毒体外感染人血管内皮细胞后,用RT-PCR法检测病毒整合细胞并复制状况,用MTT法检测细胞增殖状况,流式细胞术及免疫印迹检测细胞凋亡。结果琼脂糖电泳结果显示,H1N1病毒能感染血管内皮细胞且能复制。MTT结果显示,尽管H1N1病毒不能完全抑制血管内皮细胞的增殖,但能使细胞增殖能力明显下降。流式细胞术结果显示甲型流感病毒感染组的Annein V染色阳性的血管内皮细胞比例明显高于对照组。免疫印迹结果显示病毒感染24、32 h后,细胞出现断裂的PRAP蛋白条带。结论甲型流感病毒能感染人血管内皮细胞及增殖,并能诱导血管内皮细胞凋亡。Objective To clarify the effect of human H1N1 virus on the proliferation and apoptosis in human endothelial cells. Methods After the purified H1N1 virus infected human umbilical vein endothelial cells,virus replication was confirmed by RT-PCR method,the cellular proliferation was determined by MTT,and the apoptosis was detected by flow cytometry analysis and Western blot analysis. Results The number of influenza virus genome in the infected endothelial cells at 6 hours post-infection was increased as compared with no treatment.After being infected with H1N1 virus for 24 hours, the cellular proliferation was significantly inhibited.Many Annein-V staining positive endothelial cells were observed using flow cytometry method.Furthermore,cleaved PRAP was also detected in H1N1 virus-infected cells by Western blot. Conclusions The results show that H1N1 influenza virus can infect and replicate in human umbilical vein endothelial cells and can induce the apoptosis in them.

关 键 词：甲型流感病毒 人脐带血管内皮细胞 增殖 细胞凋亡 

分 类 号：R373.13[医药卫生—病原生物学]