栀子总环烯醚萜苷含量的紫外分光光度法测定  

Determination of Total Iridoid Glycosides in Gardenia jasminoides Ellis by Ultraviolet Spectrophotometry

作　　者：周婷婷[1,2] 闻俊[1,2] 佟典承[1,2] 范国荣[1,2] 

机构地区：[1]中国人民解放军第二军医大学,上海200433 [2]上海市药物代谢重点实验室,上海200433

出　　处：《时珍国医国药》2011年第2期273-275,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30801541);中国人民解放军第二军医大学青年人才启动基金

摘　　要：目的测定栀子药材乙醇提取物中总环烯醚萜苷的含量。方法采用紫外分光光度法。结果以栀子苷为标准品,栀子总环烯醚萜苷浓度在8.08~24.24μg.ml-1线性关系良好,方法精密度、重复性、加样回收率、稳定性均良好,3批栀子乙醇提取物中总环烯醚萜苷平均含量为56.5%。结论该方法操作简便,结果可靠,重复性好,可作为中药栀子质量控制的方法。Objective To determine the content of total iridoid glycosides in Gardenia jasminoides Ellis.Methods Ultraviolet spectrophotometry with the detective wavelength set at 238 nm was used.Results Geniposide was served as a referenc,the linear range of geniposide was 8.08~24.24 μg·ml-1（r=0.999 9） and its average recovery was 100.25%（RSD=2.83%）.The average content of total iridoid glycosides was 56.5%.Conclusion This method is simple,rapid and accurate,and suitable for the quality control of Gardenia jasminoides Ellis.

关 键 词：栀子 总环烯醚萜苷 紫外分光光度法 栀子苷 

分 类 号：R284.2[医药卫生—中药学]