板蓝根活性部位抗单纯疱疹病毒1型分子机制的研究  

Study on Anti-viral Molecular Mechanism of Active Constituent from Radix Isatidis against Herpes Simplex Virus Type 1

作　　者：董伟[1] 张军峰[1] 何立巍[1] 高峰 佟书娟[1] 詹瑧[1] 

机构地区：[1]南京中医药大学,江苏南京210046 [2]江苏省中医医院,江苏南京210029

出　　处：《时珍国医国药》2011年第2期396-398,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家教育部博士点基金课题资助项目(No.20093237120015);江苏省中医药管理局科技研究专项基金(No.HZ07044);江苏省高校自然科学基金课题资助项目(No.09KJB360006);南京中医药大学青年自然科学基金(No.09XZR02)

摘　　要：目的探讨板蓝根活性部位体外抗单纯疱疹病毒1型的分子机制。方法利用MTT法检测药物对单纯疱疹病毒感染后Vero细胞活性程度的影响。实时定量RT-PCR的方法研究药物对单纯疱疹病毒1型胸腺嘧啶核苷激酶和DNA多聚酶的影响。结果板蓝根提取部位在病毒感染早期抑制了单纯疱疹病毒的胸腺嘧啶核苷激酶和DNA多聚酶的复制。结论板蓝根活性部位在单纯疱疹病毒感染细胞的早期阶段可以降低病毒即刻早期基因——胸腺嘧啶核苷激酶和DNA多聚酶的mRNA,发挥抗病毒作用。Objective To study the anti-viral molecular mechanism of active constituent from Radix Isatidis against herpes simplex virus type 1 in vitro.Methods To detect the effect of active constituent from Radix Isatidis on the cellular activity of HSV-1 infected vero cells by methyl thiazolyl tetrazoliym assay.To research the anti-viral effect of Radix Isatidis on thymidine kinase and DNA polymerase by real time RT-PCR.Results Active constituent from Radix Isatidis decreased TK and DNA polymerase copies in early stage by real time RT-PCR.Conclusion Effective components of Radix Isatidis can reduce mRNA of some herpes simplex virus type 1 immediate early genes,such as HSV-1 thymidine kinase and DNA polymerase,and show its function against HSV-1.

关 键 词：板蓝根 单纯疱疹病毒 抗病毒 

分 类 号：R285.5[医药卫生—中药学]