氯丙嗪、尼莫地平单用和合用对小鼠镉中毒肝、肾毒效应的影响  

INFLUENCES OF CHLOROPAZINE,NIMODIPINE AND THEIR COMBINATON ON THE TOXIC EFFECTS OF CADMIUM ON LIVERS AND KIDNEYS OF MICE

作　　者：唐玲芳[1] 陈砚朦[1] 张珍玲[1] 宋玲[1] 杨永年[1] 冯致英[1] 

机构地区：[1]南京医科大学卫生毒理学教研室,南京210029

出　　处：《癌变．畸变．突变》1999年第6期334-337,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：卫生部科学研究基金;江苏省卫生厅资助

摘　　要：观察钙调素拮抗剂氯丙嗪（ Ｃ Ｐ Ｚ） 和钙离子通道阻断剂尼莫地平（ Ｎ Ｉ Ｍ Ｏ） 以及同时给予以上两药对镉（ Ｃｄ） 致小鼠肝、肾毒作用的保护作用。利用模拟镉中毒动物模型。实验组预先灌胃给予小鼠 Ｃ Ｐ Ｚ、 Ｎ Ｉ Ｍ Ｏ、 Ｃ Ｐ Ｚ＋ Ｎ Ｉ Ｍ Ｏ，１ 小时后以１／５ Ｌ Ｄ－ ５０ Ｃｄ Ｃｌ２ 腹腔注射，连续五天，第六天收集生物样本检测各项指标。结果表明， Ｃ Ｐ Ｚ 能明显降低血镉，降低尿中ｒ － Ｇ Ｔ 及 Ｎ Ａ Ｇ 活性。 Ｃ Ｐ Ｚ和 Ｎ Ｉ Ｍ Ｏ 对于镉引起的钙泵活性降低，均表现出明显的保护作用，两药合用作用增强。 Ｃ Ｐ Ｚ 和 Ｎ Ｉ Ｍ Ｏ 均能显著提高镉中毒小鼠肝、肾组织中镉金属硫蛋白（ Ｍ Ｔ） 含量。揭示这两种钙拮抗剂对小鼠镉中毒肝、肾毒效应均有防护作用。 Ｃ Ｐ Ｚ 比 Ｎ Ｉ Ｍ Ｏ 明显。这两种药物合用在某些方面起协同作用。该两药的防护作用可能与 Ｍ Ｔ 参与有关。The influences of calmodulin antagonist CPZ, and calcium channel blocker NIMO and the combination on cadmium poisoning of mice were studied. Animal models for cadmium poisoning were established by peritoneal injection of 1/5 LD\-\{50\}CdCl\-2, The experimental groups were protected by administering of CPZ\,NIMO and combination of CPZ and NIMO respectively one hour before the injection of CdCl\-2. Five consecutive days later, samples were collected for analysis. The data showed that the Cd content in blood and the urinary γ GT and NAG activities were reduced significantly by CPZ. Both CPZ and NIMO showed considerable protective effect against the decrease of Ca\+\{2+\}-Mg\+\{2+\}-ATPase activity, and a remarkable synergistic action between them was observed. Both CPZ and NIMO could considerably increase MT contents in livers and kidneys of Cd poisonig mice. The results indicated that the inhibitors could protect mice against the toxic effects of Cd on liver and kidney, while CPZ was more efficient than NIMO. The combination of CPZ and NIMO could exert a synergistic action in some aspects. The protective action of these two drugs might be relevent to the function of MT.

关 键 词：镉中毒 氯丙嗪 尼莫地平 镉金属硫蛋白 

分 类 号：R595.205[医药卫生—内科学] R995[医药卫生—临床医学]