悬铃木ISSR反应体系的建立  

ESTABLISHMENT OF ISSR REACTION SYSTEM OF PLATANUS ACERIFOLIA

作　　者：王艳梅[1] 景自景[1] 刘震[1,2] 田佳桂[1] 王佳慧[1] 

机构地区：[1]河南农业大学林学院,河南郑州450002 [2]北京林业大学林学院,森林培育与保护省部共建重点实验室,北京100083

出　　处：《山东农业大学学报（自然科学版）》2011年第1期30-34,共5页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：教育部留学回国人员科研启动资助项目(2000);教育部博士点基金项目(200804660002);河南省杰出青年基金项目(002);河南农业大学博士启动项目(30400376);河南省教育厅自然科学研究计划项目(2010A220001)

摘　　要：为了探讨适合悬铃木的ISSR体系,选育无球少球悬铃木,本实验以悬铃木幼嫩叶片为实验材料,通过对CTAB方法的改良,摸索出适于悬铃木基因组DNA提取的方法,并以核酸纯度、片断分布情况等指标来评价,获得高质量基因组DNA;用2个不同样品对16对ISSR引物进行了筛选,并应用其中的4对ISSR引物对15株悬铃木进行了初步应用,获得了清晰稳定的扩增图谱。上述结果表明获得的基因组质量较高,同时也表明反映体系对悬铃木的ISSR分析是可行的;并对影响悬铃木基因组DNA的制备及扩增反应因素进行了简要的分析讨论。To probe the effective ISSR system and to breed none - or - little cones Platanus acerifolia, genomic DNA was extracted with modified CTAB method from tender leaves of Platanus acerifolia. DNA quality was e- valuated by indexes like purity and distribution of nucleic acid. High - quality genomic DNA was obtained. Sixteen ISSR primers were screened with 2 different samples. 4 ISSR primers were adopted to evaluate preliminaryinitially among 15 plane trees and clear and stable amplification patterns was obtained,which indicated the DNA was good and the system was feasible. The factors affecting the DNA extraction and amplification of genome DNA of Platanus acerifolia were discussed in the paper.

关 键 词：悬铃木 ISSR 无球少球 

分 类 号：Q943.2[生物学—植物学]